Nickel(II) Chloride was obtained from Wako Pure Chemical Corporation. Freund’s incomplete adjuvant (IFA) and complete adjuvant (CFA) were from MP Biomedicals. The primary antibody specific for Sema3A was from Abcam. Antibodies specific for phospho-p38 (Thr180/Tyr182) and p38 were obtained from Cell Signaling Technology. Rabbit antibody to GAPDH was obtained from Osenses. Rabbit antibody to β-actin was obtained from Bioss. Horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody were obtained from Cell Signaling. FITC anti-mouse CD11c, Alexa Fluor 488 anti-mouse CD11b, PE anti-mouse F4/80, PE anti-mouse I-A/I-E, FITC anti-mouse CD3, PE/Cyanine7 anti-mouse CD4 and PE anti-mouse CD8a were purchased from BioLegend. APC-Cy™7 Rat Anti-Mouse CD45 and Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) were from BD Biosciences. 7-AAD Viability Staining Solution was from Invitrogen. Anti-rabbit Alexa Fluor 488 secondary antibody was from Abcam. ReadidropTM Propidium Iodide was from Bio-Rad Laboratories. The recombinant mouse Sema3A were purchased from R&D Systems.
Antibodies specific for phospho p38 thr180 tyr182 and p38
Manufactured by Cell Signaling Technology
The Antibodies specific for phospho-p38 (Thr180/Tyr182) and p38 are laboratory reagents used to detect and quantify the levels of phosphorylated and total p38 MAPK protein in biological samples. These antibodies are designed for use in techniques such as Western blotting, immunohistochemistry, and flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase hrp conjugated anti rabbit secondary antibody, by Cell Signaling Technology (2 mentions)
Freund s incomplete adjuvant ifa, by MP Biomedicals (2 mentions)
Complete adjuvant cfa, by MP Biomedicals (2 mentions)
Alexa fluor 488 anti mouse cd11b, by BioLegend (2 mentions)
Nickel 2 chloride, by Fujifilm (2 mentions)
Pe anti mouse 1 a 1 e, by BioLegend (2 mentions)
Pe anti mouse f4 80, by BioLegend (2 mentions)
Pe cyanine7 anti mouse cd4, by BioLegend (1 mentions)
Fitc anti mouse cd3, by BioLegend (1 mentions)
Purified rat anti mouse cd16 cd32 mouse fc block, by BD (1 mentions)
Pe anti mouse cd8a, by BioLegend (1 mentions)
Apc cy7 rat anti mouse cd45, by BD (1 mentions)
Readidroptm propidium iodide, by Bio-Rad (1 mentions)
7 aad viability staining solution, by Thermo Fisher Scientific (1 mentions)
Fitc anti mouse cd11c, by BioLegend (1 mentions)
Alexa fluor 488 secondary antibody, by Abcam (1 mentions)
Alexa fluor 488 antimouse cd11c, by BioLegend (1 mentions)
Alexa fluor 488 anti rabbit secondary antibody, by Abcam (1 mentions)
Alexa fluor 647 antimouse cd206 mmr, by BioLegend (1 mentions)
Pe anti mouse cd115, by BioLegend (1 mentions)
2 protocols using antibodies specific for phospho p38 thr180 tyr182 and p38
1
Sema3A Signaling in Immune Responses
2
Sema3A Regulation and Immune Response
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Nickel(II) Chloride was obtained from Wako Pure Chemical Corporation. Freund's incomplete adjuvant (IFA) and complete adjuvant (CFA) were from MP Biomedicals. The primary antibodies specific for Sema3A were from Bioss Antibodies (for Western blotting) and Abcam (for immunohistochemistry/immunocytochemistry), respectively. Antibodies specific for phospho-p38 (Thr180/Tyr182) and p38 were obtained from Cell Signaling Technology. Antibody specific for mouse TSLP was from Abcam. Rabbit antibody to GAPDH was obtained from Osenses. Alexa Fluor 488 anti-mouse CD11c, Alexa Fluor 488 anti-mouse CD11b, PE anti-mouse CD115, PE anti-mouse F4/80, PE anti-mouse I-A/I-E and Alexa Fluor 647 anti-mouse CD206 (MMR) were purchased from BioLegend. Horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody were obtained from Cell Signaling. Anti-rabbit Alexa Fluor 488 secondary antibody was from Abcam.
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