Mapk family antibodysampler kit 9926t

Manufactured by Cell Signaling Technology

The MAPK Family AntibodySampler Kit 9926T contains a collection of antibodies targeting members of the mitogen-activated protein kinase (MAPK) family. This kit provides researchers with a convenient way to detect and study the expression and activation of key MAPK proteins, which play crucial roles in cellular signaling pathways.

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Lab products found in correlation

Phospho mapk family antibody sampler kit9910t, by Cell Signaling Technology (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Yeasen (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) Mito tracker, by Yeasen (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Yeasen (1 mentions) Annexin 5 fitc pi, by Yeasen (1 mentions) Fluo 4 am, by Yeasen (1 mentions) Calcein am, by Yeasen (1 mentions) Hoechst 33342, by Yeasen (1 mentions) P nf bp65, by Cell Signaling Technology (1 mentions) Recombinant mouse m csf, by R&D Systems (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Cell culture medium, by Thermo Fisher Scientific (1 mentions) Trap staining kit, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rankl, by R&D Systems (1 mentions)

2 protocols using mapk family antibodysampler kit 9926t

Multimodal Cytotoxicity Assay Protocol

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MTT, calcein-AM, EthD-1, AnnexinV-FITC/PI, ER-tracker, Mito-Tracker, Fluo-4-AM,
DAPI, Hoechst 33342, H2DCF-DA, MEQ, and BCECF-AM were purchased from Yeasen
(Shanghai, China). Z-VAD-fmk, 3-methyladenine (3-MA), bafilomycinA1 (Baf),
cycloheximide (CHX), N-acetylcysteine (NAC), reduced
glutathione (GSH), SB203580, U0126, SP600125, ruthenium red (RR), and disodium
4,4′-diisothiocyanato-2,2′-stilbenedisulfonate hydrate (DIDS) were obtained from
Selleck Chemicals (Houston, TX). The following antibodies were used: Cleaved
Caspase Antibody Sampler Kit 9929T (cleaved caspase-3, -7, and -9 and cleaved
poly-ADP-ribose polymerase [PARP]), Procaspase Antibody Sampler Kit 12742T
(Caspase-3, -7, and -9; PARP), Bcl-2 (3498T), Bax (2772T), Beclin-1(3495T),
LC3I/II (12741T), p62(5114S), ER Stress Antibody Sampler Kit 9956T (calnexin,
BiP, IRE1α, CHOP), ubiquitin (43124S), XBP1s (83418S), MAPK Family Antibody
Sampler Kit 9926T (ERK 1/2, p38, JNK), Phospho-MAPK Family Antibody Sampler Kit
9910T (phospho-ERK1/2, phospho-p38, phospho-JNK, Rabbit IgG HRP, Mouse IgG HRP),
GAPDH (5174S), and chloride intracellular channel-1 (CLIC1; 53424S) were
purchased from Cell Signaling Technology (Danvers, MA). Alexa Fluor 647
AffiniPure Goat anti-Rabbit immunoglobulin G (IgG; H + L) (cat: FMS-RBaf64701)
was obtained from FCMRCS (Nanjing, China).

Zhu D., Chen C., Xia Y., Kong L.Y, & Luo J. (2019). A Purified Resin Glycoside Fraction from Pharbitidis Semen Induces Paraptosis by Activating Chloride Intracellular Channel-1 in Human Colon Cancer Cells. Integrative Cancer Therapies, 18, 1534735418822120.

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Osteoclastogenesis Assay Protocol

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Mon was purchased from Solarbio (Beijing, China). Fetal bovine serum (FBS) and cell culture medium were obtained from Gibco-BRL (Sydney, Australia). Recombinant mouse M-CSF and RANKL were purchased from R&D Systems (Minneapolis, MN, USA). TRAP staining kit was obtained from Sigma (St. Louis, MO, USA). TRIZOL reagent was bought from Invitrogen. PrimeScript TM RT reagent Kit was obtained from TaKaRa Biotechnology. RAW264.7 cells were purchased from Cell Bank (Chinese Academy of Sciences, Shanghai, China). The inhibitors of protease and phosphatase were purchased from Solarbio (Beijing, China). Antibodies for p-p38, p38, p-JNK, JNK, p-ERK, ERK, p-NF-ĸB p65, NF-ĸB p65, IĸBα and β-actin were obtained from Cell Signaling Technology (MAPK Family Antibody Sampler Kit #9926T, Phospho-MAPK Family Antibody Sampler Kit #9910T, and NF-κB Family Antibody Sampler Kit #9936).

Wei C.M., Su Y.J., Qin X., Ding J.X., Liu Q., Song F.M., Zong S.H., Xu J., Zhou B, & Zhao J.M. (2018). Monocrotaline Suppresses RANKL-Induced Osteoclastogenesis In Vitro and Prevents LPS-Induced Bone Loss In Vivo. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 48(2).

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