Dmem with and without phenol red

BT-474 and MDAMB231 were purchased by ATCC (Manassas, VA, USA). DMEM (with and without phenol red) and fetal calf serum were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bradford protein assay kit, as well as anti-mouse secondary antibodies, were obtained from Bio-Rad (Hercules, CA, USA). Antibodies against ERα (F-10, mouse) and ubiquitin (P4D1, mouse) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-vinculin (mouse) antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA). Chemiluminescence reagent for Western blotting was obtained from BioRad Laboratories (Hercules, CA, USA). Fulvestrant (i.e., ICI182,780) was purchased by Tocris (USA). All the other products were from Sigma-Aldrich. Analytical- or reagent-grade products were used without further purification. The identity of the BT-474 and MDAMB231 cell lines was verified by STR analysis (BMR Genomics, Italy).

17β-estradiol, DMEM (with and without phenol red) and fetal calf serum were purchased from Sigma-Aldrich (St. Louis, MO). The Bradford protein assay kit, as well as anti-mouse and anti-rabbit secondary antibodies, were obtained from Bio-Rad (Hercules, CA). Antibodies against ERα (HC-20 rabbit; F-10 mouse), cyclin D1 (H-295 rabbit), Bcl-2 (C2 mouse), progesterone receptor (C20 rabbit), cathepsin D (H75 rabbit), pS2 (FL-84 rabbit), dynamin II (C-18 goat), p62/SQSTM (D-3 mouse) and anti-goat secondary antibody (sc-2020) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and anti-vinculin, anti-tubulin and anti-LC3 antibodies were purchased from Sigma-Aldrich (St. Louis, MO). Anti-phospho-AKT, anti-AKT, anti-phospho-S118 ERα, and anti-IGF-1R were purchased from Cell Signaling Technology, Inc. (Beverly, MA). Anti-biotin-HRP was purchased from Thermo Fisher Scientific (Walthman, MA, USA). Chemiluminescence reagent for Western blotting was obtained from BioRad Laboratories (Hercules, CA, USA). Dynole 2–24 was purchased from Abcam (USA). All other products were from Sigma-Aldrich. Analytical- or reagent-grade products, without further purification, were used. The identities of all of the cell lines that were used (i.e., human breast carcinoma cells [MCF-7; T47D-1]) were verified by STR analysis (BMR Genomics, Italy).