Rna interference sequences

Ti nanoparticles were obtained from Nanjing Emperor Nano Materials Company. RNA interference sequences were purchased from GenePharma (Suzhou, China). Murine RANKL was purchased from R&D Systems (Minneapolis, USA). GKT137831 was purchased from ApexBio (Boston, USA). Dulbecco’s modified Eagle’s medium (DMEM/high glucose) was purchased from VivaCell (Shanghai, China) and fetal bovine serum (FBS) was obtained from HyClone (Logan, USA). CCK-8 assay kits were obtained from ApexBio (Boston, USA). The primary antibodies used in our study included NFATc1 (A1539, ABclonal, Wuhan, China), MMP-9 (A0289, ABclonal), NOX4 (A11274, ABclonal), HO-1 (ab189491, Abcam Cambridge, UK), SOD2 (ab137037, Abcam), and Nrf2 (A0674, ABclonal). Secondary antibody was purchased from Multisciences (Hangzhou, China).

Ti particles were purchased from Alfa Aesar (Ward Hill, MA). Recombinant RANKL was obtained from R&D Systems (Minneapolis, USA). RAW264.7 cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). RNA interference sequences were obtained from GenePharma (Suzhou, China). DMEM/high glucose and fetal bovine serum (FBS) were bought from Thermo Fisher Scientific (St. Louis, MO, USA). ML171 was bought from the Master of Bioactive Molecules (Suzhou, China). AM630 was bought from Beyotime (Shanghai, China). The primary antibodies employed in our experiment included CTSK (A5871, ABclonal, Wuhan, China), NFATc1 (A1539, ABclonal), MMP9 (A0289, ABclonal), ACP5 (A2528, ABclonal), NOX1 (A8527, ABclonal), NOX2 (A19701, ABclonal) and β-Actin (AC006, ABclonal). The ROS Assay Kit was bought from Beyotime (Shanghai, China).