Anti human cd14 phycoerythrin pe cy7

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Anti-human CD14-phycoerythrin (PE) Cy7 is a fluorochrome-conjugated monoclonal antibody that binds to the CD14 antigen expressed on the surface of human monocytes, macrophages, and some dendritic cells. It can be used for the identification and enumeration of these cell types in flow cytometry applications.

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Spelling variants of this product

CD14-PE (35 citations) CD14 PE-Cy7 (16 citations) Anti-CD14-PE (8 citations) Anti-human CD14-PE/Cy7 (5 citations) Phycoerythrin (PE) (5 citations)

2 protocols using anti human cd14 phycoerythrin pe cy7

Immune Phenotyping of T Cells and Dendritic Cells in Kawasaki Disease

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Given the role of T cells and myeloid dendritic cells (DC) in the pro- and anti-inflammatory pathways in KD, we focused on immune phenotyping these cell populations. Myeloid DC populations were characterized and enumerated by flow cytometry using the following monoclonal antibodies (mAb) : anti-human CD11c-APC, mouse IgG1κ, clone B-ly6, anti-human CD11b-APC-Cyanin 7 (Cy7), mouse IgG1κ, clone ICRF44, anti-human CD14-phycoerythrin (PE) Cy7, mouse IgG2aκ, clone M5E2, anti-human CD86-fluorescein isothiocyanate (FITC), mouse IgG1κ, clone 2331 (FuN-1) (eBioscience).
T cell populations were characterized and enumerated by flow cytometry using the following mAb: CD25 BV421, mouse IgG1 k, clone M-A251 (BD Bioscience), anti-human CD4-percp-Cy5.5, mouse IgG1k, clone RPA-T4, anti-human CD8, Alexa Fluor 700, clone RPA-T8, mouse IgG1k, anti-human CD45RA APC, mouse IgG2b k, clone HI100, anti-human CD127 FITC, mouse IgG1 k, clone eBioRDR5, anti-human HLA-DR APC-H7 clone G46-6, mouse IgG2a k (eBioscience). Data were acquired with FACS ARIA II and analyzed using FACSDiva (BS Biosciences, San Jose, CA).

Tremoulet A.H., Jain S., Jone P.N., Best B.M., Duxbury E.H., Franco A., Printz B., Dominguez S.R., Heizer H., Anderson M.S., Glodé M.P., He F., Padilla R.L., Shimizu C., Bainto E., Pancheri J., Cohen H.J., Whitin J.C, & Burns J.C. (2019). Phase I/IIa Trial of Atorvastatin in Patients With Acute Kawasaki Disease With Coronary Artery Aneurysms. The Journal of pediatrics, 215, 107-117.e12.

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Myeloid Dendritic Cell Immunophenotyping

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Myeloid dendritic cells (mDC) and macrophage populations and their maturation stage were defined by binding of the following monoclonal antibodies (mAb) to cell surface markers and analyzed by flow cytometry: anti‐human CD11c allophycocyanin (APC), mouse immunoglobulin (Ig)G1κ, clone B‐ly6, anti‐human CD11b APC/cyanin 7 (Cy7), mouse IgG1κ, clone ICRF44, anti‐human CD14 phycoerythrin (PE)/Cy7, mouse IgG2aκ, clone M5E2, anti‐human CD86 fluorescein isothiocyanate (FITC), mouse IgG1κ, clone 2331 (FuN‐1), anti‐human CD4 AF700 mouse IgG1κ, clone RPA‐T4, anti‐human ILT‐4 peridinin chlorophyll (PerCp)/eF710 and mouse IgG2bκ, clone 42D1 from eBioscience (San Diego, CA, USA). Data for all immunophenotyping were acquired with fluorescence activated cell sorter (FACS) ARIA II and analyzed using FACSDiva (BD Biosciences, San Jose, CA, USA) software. Normal pediatric ranges for mDC and tolerogenic mDC (tmDC) were previously determined by our group [5, 6].

Burns J.C., Hsieh L.E., Kumar J., Behnamfar N., Shimizu C., Sivilay N., Tremoulet A.H, & Franco A. (2020). Characterization of circulating immune cells in acute Kawasaki disease suggests exposure to different antigens. Clinical and Experimental Immunology, 202(3), 263-272.

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