Antibodies against Gbp2 (ab203238), GFAP (ab53554, ab7260), LCN2 (ab63929), LRP1 (ab92544), MAG (ab89780), MBP (ab40390), NF200 (ab7795), PLP (ab28486) and S100A10 (ab76472) were purchased from Abcam, UK; antibody against CD31 (550274) was purchased from BD Biosciences, USA; antibodies against GFAP (3670S), GFP (2955S, 2956S), p38 (9212S), pp38 (4511S) and β-actin (8457S) were purchased from Cell Signaling Technology, USA; antibodies against CC1 (OP80), dMBP (AB5864) and Olig2 (AB9610) were purchased from Millipore, USA; antibodies against C3d (AF2655), Galectin3 (AF1197), LAMP1 (AF4320) and LCN2 (AF1857) were purchased from R&D Systems, USA; antibodies against Gbp2 (sc-166960) and LRP1 (sc-57351) were purchased from Santa Cruz Biotechnology, USA; antibody against pLRP1 (PA5-101013) was purchased from Thermo Fisher, USA; antibody against Iba1 (019-19741) was purchased from Wako, Japan. All antibodies were used at a dilution of 1:50 to 1:2000 for immunofluorescence and 1:800 to 1:5000 for immunoblotting according to the manufacturer’s instructions. Alexa Fluor 488, 594 and 647 conjugated secondary antibodies were purchased from Jackson, USA.
Ab76472
Manufactured by Abcam
Sourced in United Kingdom
Ab76472 is a laboratory instrument used for performing analytical procedures. The core function of this product is to facilitate specific experimental tasks within a research or testing environment.
Automatically generated - may contain errors
Lab products found in correlation
Ab7795, by Abcam (1 mentions)
Ab89780, by Abcam (1 mentions)
Ab28486, by Abcam (1 mentions)
Ab40390, by Abcam (1 mentions)
Ab63929, by Abcam (1 mentions)
Sc 57351, by Santa Cruz Biotechnology (1 mentions)
β actin 8457, by Cell Signaling Technology (1 mentions)
Af1857, by R&D Systems (1 mentions)
Sc 166960, by Santa Cruz Biotechnology (1 mentions)
Ab9610, by Merck Group (1 mentions)
Ab15580, by Abcam (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
Ab205718, by Abcam (1 mentions)
Chemicdoc xrs system, by Bio-Rad (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Ab9485, by Abcam (1 mentions)
Polyvinylidene difluoride membrane, by Bio-Rad (1 mentions)
Ab115777, by Abcam (1 mentions)
Ab275018, by Abcam (1 mentions)
Anti s100a10, by Abcam (1 mentions)
5 protocols using ab76472
1
Comprehensive Antibody Validation Protocol
2
Immunohistochemical Analysis of Ki67 and S100A10
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Tissue sections were prepared and subjected to immunohistochemical analysis. Anti-human Ki67 (ab15580, Abcam), S100A10 (ab76472, Abcam) antibody was used as primary antibodies. HRP-conjugated secondary Ab was used as secondary antibody. The images were captured by Olympus-BX51 microscope (Olympus, Japan).
3
Protein Expression Analysis by Western Blot
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Total proteins were prepared with RIPA lysis buffer (Beyotime, China). Concentrations of protein were examined by BCA assay. Equal amounts of protein were separated by 10% SDS-PAGE and electrotransferred onto polyvinylidene difluoride membranes (Bio-Rad, United States). After being blocked using 5% non-fat milk for 1 h at room temperature, the primary rabbit anti-human antibodies against S100A10 (ab76472, Abcam) and GAPDH (ab9485, Abcam) were supplemented overnight at 4°C. Then the members were washed with tris-buffered saline Tween 20 for three times and probed with horse radish peroxidase-conjugated secondary antibodies (ab205718, Abcam) for 1 h at 37°C. The bands were visualized using a ChemicDocXRS system (Bio-Rad, United States).
4
Protein Extraction and Western Blotting
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The cells were lysed with RIPA lysate (containing 1% PMSF), and the supernatant was collected after the high-speed centrifugation. Subsequently, the Bradford method was used for protein quantification. Then the sample was heated in a water bath at 100°C for 10 min. SDS-PAGE was used to separate the proteins and then the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes. After that, the membrane was blocked at room temperature with 5% skimmed milk for 1 h, followed by being added with anti-Anti-APE1 antibody (ab76472) (1:1000; Abcam, Cambridge, UK) and Anti-β-actin antibody (ab115777) (1:1000; Abcam, Cambridge, UK) for incubation overnight at 4°C and then rinsed with TBST solution. Subsequently, the membrane was incubated with secondary antibodies (Beyotime, Shanghai, China) for 1 h at room temperature before being washed 3 times. At last, color rendering was performed using hypersensitive ECL (Biossci Biotechnology Co, Ltd., Wuhan, China).
5
Western Blot Analysis of Neuronal Markers
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Western blot analysis was performed as previously reported (Jiang et al., 2016 (link); Bier et al., 2018 (link)). Briefly, cell lysates were solubilized with RIPA buffer supplemented with PhosSTOP and Complete Phosphatase/Protease Inhibitor Cocktails (Roche Diagnostics) and protein content was analyzed using a standard BCA assay. Protein extract (20–30 μg per sample) were loaded on sodium dodecyl sulfate-polyacrylamide electrophoresis gels and transferred to PVDF membranes that were probed with the specific antibodies as detailed. Bound antibodies were visualized with an enhanced chemiluminescence detection kit (Amersham Pharma-Biotech). Equal loading was verified using an anti actin antibody. The following primary antibodies were used: Anti-TrkB (Cat# sc-136990, 1:500), anti-p75 (Cat# sc-13577, 1:1,000), and anti-EAAT2 antibodies (Cat# sc-365634, 1:500) (Santa Cruz Biotechnology). Anti-cleaved PARP1 (AB3820, 1:1,000), Anti-cleaved caspase 3 (ab2303, 1:500), anti-C3 (ab97462, 1:500) anti-S100A10 (ab76472, 1:500) and exosome panel antibody (ab275018) (Abcam, Cambridge, MA United States). Anti-mouse and anti-rabbit HRP secondary antibodies (1:10,000, Pierce).
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