All organic solvents (methanol, ethanol, acetone, dimethyl formamide (DMF) and, N,N-diisopropylethylamine (DIPEA)) were HPLC grade (Fisher Scientific) and used without further purification. E. coli cells (BL21 and NEB 5-alpha) were purchased from New England Biolabs. Lysozyme and Sephadex LH-20 were purchased from Sigma-Aldrich. BLUEStain protein ladder and ampicillin were purchased from Gold Biotechnology. Yeast extract, L-proline, tryptone, glycerol, and other bacterial culture media components were purchased from Research Products International. All materials for SDS-PAGE were obtained from Bio-Rad. PE/Cyanine7 anti-human EGFR antibody was purchased from BioLegend. Cy5.5 NHS ester was obtained from Lumi Probe. DMEM/F12, DMEM, fetal bovine serum (FBS), PenStrep, and other cell culture reagents were obtained from Corning Life Sciences.
Tryptone
Manufactured by Research Products International
Tryptone is a complex mixture of peptides and amino acids derived from the enzymatic digestion of casein. It is commonly used as a source of nitrogen, carbon, and other nutrients in microbiological growth media, supporting the growth of a variety of microorganisms. Tryptone provides essential nutrients for the cultivation of bacteria, fungi, and other microbes in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Yeast extract, by Research Products International (2 mentions)
Ampicillin, by GoldBio (1 mentions)
Neb 5 alpha, by New England Biolabs (1 mentions)
Glycerol, by Research Products International (1 mentions)
Bluestain protein ladder, by GoldBio (1 mentions)
Lysozyme, by Merck Group (1 mentions)
Sephadex lh 20, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Dmem f12, by Corning (1 mentions)
Cy5.5 nhs ester, by Lumiprobe (1 mentions)
Fmoc trp boc oh, by Merck Group (1 mentions)
Magnesium sulfate heptahydrate, by Thermo Fisher Scientific (1 mentions)
Tetracycline hydrochloride, by Thermo Fisher Scientific (1 mentions)
Imidazole, by Thermo Fisher Scientific (1 mentions)
Lysozyme, by Thermo Fisher Scientific (1 mentions)
Kanamycin sulfate, by IBI Scientific (1 mentions)
Criterion tgx stain free protein gel, by Bio-Rad (1 mentions)
Triton x 100, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Deoxynucleotides, by New England Biolabs (1 mentions)
4 protocols using tryptone
1
Protein Expression and Purification Protocol
2
Preparation of Tryptophan-Containing Biomolecules
3
Purification and Characterization of Protein
4
Purification and Characterization of StsA
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Materials and reagents were purchased from
Gold Biotechnology, Fisher Scientific, or Sigma-Aldrich unless otherwise
noted. Yeast extract and tryptone were purchased from Research Products
International. Molecular biology reagents for cloning (e.g., restriction
enzymes, Q5 polymerase, T4 DNA ligase, and deoxynucleotides) were
purchased from New England Biolabs. Oligonucleotide primers and gene
blocks were obtained from Integrated DNA Technologies. DNA spin columns
were purchased from Epoch Life Sciences. Sanger sequencing was performed
by the Roy J. Carver Biotechnology Center (University of Illinois
at Urbana-Champaign). Polymerase chain reactions were performed using
a Bio-Rad S1000 thermal cycler. Escherichia coli DH5α and BL21(DE3) strains were used for plasmid maintenance
and protein overexpression, respectively. Expressed StsA was purified
using an Agilent 1200 series HPLC fitted with a 10 × 250 mm C18
column (Macherey Nagel). Mass spectroscopy was performed using a Bruker
Daltonics UltrafleXtreme MALDI-TOF mass spectrometer and a ThermoFisher
Scientific Orbitrap Fusion ESI-MS using an Advion TriVersa Nanomate
100.
Gold Biotechnology, Fisher Scientific, or Sigma-Aldrich unless otherwise
noted. Yeast extract and tryptone were purchased from Research Products
International. Molecular biology reagents for cloning (e.g., restriction
enzymes, Q5 polymerase, T4 DNA ligase, and deoxynucleotides) were
purchased from New England Biolabs. Oligonucleotide primers and gene
blocks were obtained from Integrated DNA Technologies. DNA spin columns
were purchased from Epoch Life Sciences. Sanger sequencing was performed
by the Roy J. Carver Biotechnology Center (University of Illinois
at Urbana-Champaign). Polymerase chain reactions were performed using
a Bio-Rad S1000 thermal cycler. Escherichia coli DH5α and BL21(DE3) strains were used for plasmid maintenance
and protein overexpression, respectively. Expressed StsA was purified
using an Agilent 1200 series HPLC fitted with a 10 × 250 mm C18
column (Macherey Nagel). Mass spectroscopy was performed using a Bruker
Daltonics UltrafleXtreme MALDI-TOF mass spectrometer and a ThermoFisher
Scientific Orbitrap Fusion ESI-MS using an Advion TriVersa Nanomate
100.
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