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Poloxamer 407 px 407

Manufactured by BASF
Sourced in Germany

Poloxamer 407 (PX 407) is a non-ionic surfactant and emulsifier commonly used in laboratory applications. It is a triblock copolymer composed of a central hydrophobic block of polyoxypropylene (polypropylene glycol) flanked by two hydrophilic blocks of polyoxyethylene (polyethylene glycol). Poloxamer 407 is soluble in water and can be used to modify the physical properties of solutions, suspensions, and other formulations.

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2 protocols using poloxamer 407 px 407

1

Sunflower Lecithin-Based Nanoparticle Formulation

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We purchased sunflower lecithin (lecithin) from Now Foods (Bloomingdale, IL, USA); chlorophyll-a paste from Shandong Lanmo Biotech Co., Ltd. (Changle, China); cholesterol, acetone, and dimethyl sulfoxide (DMSO) from Samchun (Pyeongtaek, Korea); Span® 80 (SP 80), Span® 20 (SP 20), and Tween® 80 (TW 80) from Dae Jung Co. Ltd. (Busan, Korea); Poloxamer 407 (PX 407) from BASF (Ludwigshafen, Germany); phosphate-buffered saline (PBS), methylene blue (MB), and chloroform from Sigma-Aldrich (St. Louis, MO, USA); methylene chloride (CH2Cl2, MC) from Duksan Co. Ltd. (Gyeonggi-do, Korea); DPBF from TCI Chemical (Tokyo, Japan); Dulbecco’s modified Eagle medium from WelGENE (Gyeongsan, South Korea); and penicillin-streptomycin solution 100× and foetal bovine serum from BioWest (Nuaillé, France). Cancer cell lines (HeLa and A549) were purchased from the Korea Cell Line Bank (Seoul, Korea). The Quanti-MAX WST-8 assay kit (WST) was purchased from Biomax (Seoul, Korea). Methanol (MeOH) of high-performance liquid chromatography (HPLC) grade was purchased from Honeywell (Seelze, Germany). All other chemicals used were of HPLC grade.
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2

Formulation and Evaluation of Lipid Nanocarriers

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Cur and phosphate-buffered saline (PBS) were
purchased from Sigma-Aldrich (St. Louis, MO, USA). Lauric acid (LA),
palmitic acid (PA), and stearic acid (SA) were supplied by SAMCHUN
(Pyeongtaek, Korea). Poloxamer 188 (PX 188) and poloxamer 407 (PX
407) were purchased from BASF (Ludwigshafen, Germany). Tween 20 (TW
20) and Tween 80 (TW 80) were purchased from Dae Jung Co., Ltd. (Busan,
Korea). Dulbecco’s modified Eagle medium (DMEM) was supplied
by WelGENE (Gyeongsan, South Korea). Penicillin–streptomycin
solution (100×) and fetal bovine serum (FBS) were purchased from
BioWest (Nuaillé, France). The cancer cell lines (HeLa, A549,
and CT-26) were purchased from the Korea Cell Line Bank (Seoul, Korea).
The Quanti-MAX WST-8 assay kit was purchased from Biomax (Seoul, Korea).
High-performance liquid chromatography (HPLC) grade methanol (MeOH)
was purchased from Honeywell (Seelze, Germany). All compounds are
>95% pure by HPLC analysis.
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