Horseradish peroxidase conjugated goat anti mouse rabbit secondary antibodies

Manufactured by Promega

Sourced in United States

Horseradish peroxidase-conjugated goat anti-mouse/rabbit secondary antibodies are laboratory reagents used in immunoassays and immunodetection techniques. They provide a means to detect and visualize target proteins or antigens that have been recognized by primary antibodies raised in mouse or rabbit. The horseradish peroxidase enzyme conjugated to the secondary antibody catalyzes a color-producing reaction, enabling the visualization and quantification of the target of interest.

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Lab products found in correlation

Fibronectin 610077, by BD (1 mentions) Phalloidin tetramethyl rhodamine b isothiocyanate p1951, by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) Gapdh, by Proteintech (1 mentions)

Close spelling variants of this product

Anti-rabbit peroxidase-conjugated secondary antibodies Secondary horseradish peroxidase conjugated antibodies Anti-mouse secondary antibodies Horseradish peroxidase

2 protocols using horseradish peroxidase conjugated goat anti mouse rabbit secondary antibodies

Immunofluorescence and Cell Assays Protocol

Cited 2 times

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The following antibodies were used in this study: SHROOM2 (SAB2700199), Vinculin (V9131) and Phalloidin–Tetramethylrhodamine B isothiocyanate (P1951) were from Sigma; E-Cadherin (24E10), N-Cadherin (13116), MLC2 (3672), p-MLC2 (T18/S19) (3674), HA (3724) were from Cell Signaling Technology; ROCK1 (ab58305), CK5 (ab52635), CK10 (ab76318), Desmoglein1 (ab12077) and RAC1 (ab155938) were from abcam; p-MYPT (ABS45) was from Millipore and Fibronectin (610077) was from BD biosciences. Horseradish peroxidase-conjugated goat anti-mouse/rabbit secondary antibodies were purchased from Promega.
Immunofluorescence microscopy, cell proliferation, colony formation, soft agar assays were performed as described previously^{8 (link),42 (link)}.

Yuan J., Chen L., Xiao J., Qi X.K., Zhang J., Li X., Wang Z., Lian Y.F., Xiang T., Zhang Y., Chen M.Y., Bei J.X., Zeng Y.X, & Feng L. (2019). SHROOM2 inhibits tumor metastasis through RhoA–ROCK pathway-dependent and -independent mechanisms in nasopharyngeal carcinoma. Cell Death & Disease, 10(2), 58.

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Antibody Panel for EMT Markers

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The following antibodies were used in this study: OVOL2 (NBP1-88754; Novus, Littleton, CO, USA), E-cadherin (24E10; Cell Signaling Technology, Danvers, MA, USA), N-cadherin (13116; Cell Signaling Technology, Danvers, MA, USA), Vimentin (5741; Cell Signaling Technology, Danvers, MA, USA), ZEB1 (sc-10572; Santa Cruz Biotechnology, CA, USA), GAPDH (60004-1-1g; Protein-tech, Chicago, IL, USA) , β-tubulin (2128S; Cell Signaling Technology, Danvers, MA, USA) and FLAG (F3165; Sigma, USA). Horseradish peroxidase-conjugated goat anti-mouse/rabbit secondary antibodies were purchased from Promega (Madison, WI, USA).

Qi X.K., Han H.Q., Zhang H.J., Xu M., Li L., Chen L., Xiang T., Feng Q.S., Kang T., Qian C.N., Cai M.Y., Tao Q., Zeng Y.X, & Feng L. (2018). OVOL2 links stemness and metastasis via fine-tuning epithelial-mesenchymal transition in nasopharyngeal carcinoma. Theranostics, 8(8), 2202-2216.

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