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Alliance 2795 lc

Manufactured by Waters Corporation

The Alliance 2795 LC is a high-performance liquid chromatography (HPLC) system designed and manufactured by Waters Corporation. It is a modular system that combines a quaternary solvent delivery system, an autosampler, a column compartment, and a variety of detection options to provide a comprehensive solution for analytical separations.

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2 protocols using alliance 2795 lc

1

Acylcarnitine Metabolite Analysis in Retina

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Acylcarnitine metabolites were extracted from WT and Vldlr−/− (P12) flash frozen retinas using ice-cold methanol. Samples were sonicated, centrifuged and the supernatant was transferred to a fresh tube for nitrogen evaporation. When dry, butanolysis was performed (butanol-HCl, 55°C for 20 min) prior to reconstitution in mobile phase (ACN:H2O 80:20, formic acid 0.05%). Samples were analyzed by liquid chromatography followed by tandem mass spectrophotometry (LC/MS/MS, Alliance 2795 LC and Quattro micro, Waters Corp). Data were recorded in positive electrospray ionization and analyzed with Neolynx (Waters Corp).
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2

Acylcarnitine Metabolite Analysis in Retina

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acylcarnitine metabolites were extracted from WT and Vldlr−/− (P12) flash frozen retinas using ice-cold methanol. Samples were sonicated, centrifuged and the supernatant was transferred to a fresh tube for nitrogen evaporation. When dry, butanolysis was performed (butanol-HCl, 55°C for 20 min) prior to reconstitution in mobile phase (ACN:H2O 80:20, formic acid 0.05%). Samples were analyzed by liquid chromatography followed by tandem mass spectrophotometry (LC/MS/MS, Alliance 2795 LC and Quattro micro, Waters Corp). Data were recorded in positive electrospray ionization and analyzed with Neolynx (Waters Corp).
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