A ~200 mg aliquot of each sSOA was weighed and extracted in an accelerated pressurized solvent extractor (Buchi SpeedExtractor E-914) according to the US EPA Method 3545A modified for in-cell extract clean-up (more details in Supplemental Materials). Extraction recovery averaged 102% (1 SD = 31.7). The resulting 40 mL DCM-solution was concentrated in a Buchi Syncore Analyst and transferred to n-hexane producing a 1 mL hydrocarbon extract. The extracts were analyzed on an Agilent 7890A gas chromatograph J&W (Agilent Select PAH GC Column (30 m × 0.25 mm × 0.15 µm)) equipped with an Agilent 7000 Triple Quadrupole mass spectrometer. Alkanes from 10 to 40 carbons in length (Table S1 ) and 36 aromatic hydrocarbons (Table S2 ) were evaluated. The PAHs included the 16 US EPA priority compounds and 11 additional PAHs listed in the International Agency for Research on Cancer (IARC). First order kinetics was applied for calculating decay rates. The Limit of Detection (LOD) ranged from 3 to 300 ng cm−3 for n-alkanes and 0.2 to 14 ng cm−3 for PAHs. Tables S1 and S2 list the MRM method parameters after optimization with the Agilent method optimization software.
7000 triple quadrupole
Manufactured by Agilent Technologies
Sourced in United States
The 7000 Triple Quadrupole is a high-performance mass spectrometry instrument designed for quantitative and qualitative analysis. It features three quadrupole mass analyzers that work in tandem to provide enhanced sensitivity and selectivity for complex sample analysis.
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Lab products found in correlation
2 protocols using 7000 triple quadrupole
1
Accelerated Pressurized Extraction and GC-MS Analysis of Alkanes and PAHs
2
GC-MS Analysis of Volatile Metabolites
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A 7890B Agilent Technologies GC system with a 7000 triple quadrupole (Agilent Technologies, United States) was used for GC-MS analysis. Separation of volatile metabolites was carried out in a ZB-5MS column (30 m length, 0.25 mm i.d., 0.25 µm film 95% dimethyl/5% diphenylpolysiloxane) from Phenomenex (United States). The injection volume was 1 µL, and the splitless mode was used. The helium carrier gas flow rate was set at 1 mL min -1 . The temperature gradient started at 60 °C in 1 min and was increased to 320 °C at an 8 °C min -1 rate. The duration of each run was 37.5 min. Additional parameters are presented in the ESI. † First, two heptane blank samples were analysed, followed by an extraction blank (the whole sample preparation procedure was the same but without the tissue homogenate), and an n-alkane standard solution (C10-C40). Then, the system was equilibrated with 10 QCs. Finally, GIST samples were injected in a randomised order, with a QC sample being injected at constant intervals.
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