Normal, decellularized and recellularized mouse ovaries (n = 3 per group) were processed for scanning electron microscopy (SEM). Due to the absence of cellular lipid membranes in the decellularized tissues, better contrast was obtained in the specimen for SEM after they were treated with osmium tetroxide and thiocarbohydrazide [37 (link)]. The treated samples were dehydrated, mounted, sputter coated with 4 nm gold particles and imaged with a Zeiss Gemini-2 SEM 500 (Carl Zeiss AB, Stockholm, Sweden).
Gemini 2 sem 500
Manufactured by Zeiss
Sourced in Germany
The Zeiss Gemini-2 SEM 500 is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of materials. It features a field emission electron source, advanced optics, and a range of detectors to provide detailed information about the surface structure and composition of samples.
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Lab products found in correlation
2 protocols using gemini 2 sem 500
1
Scanning Electron Microscopy of Ovarian Tissues
2
Collagen Fiber Quantification in Decellularized Tissue
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Samples assessed by scanning electron microscopy included discs of decellularized-, MMPs treated-, and recellularized tissue. Each specimen was prepared using the osmium tetroxide and thiocarbohydrazide method64 (link), and was imaged with Zeiss Gemini-2 SEM 500 (Carl Zeiss AG, Oberkochen, Germany). The quantification of collagen fiber thickness was performed on images taken at ×50,000 magnification. Each image was opened in ImageJ, the scale was set and the measuring tool in the software was used to measure the width of 25 individual collagen fiber strands in each image.
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