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High32capacity cdna reverse transcription kit with rnase inhibitor

Manufactured by Thermo Fisher Scientific
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The High32Capacity cDNA Reverse Transcription Kit with RNase Inhibitor is a laboratory tool designed for the reverse transcription of RNA into complementary DNA (cDNA). The kit includes reagents and enzymes necessary for the process, as well as an RNase inhibitor to protect the RNA sample.

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Lab products found in correlation

Quick rna microprep, by Zymo Research (2 mentions) Quick rna miniprep, by Zymo Research (1 mentions) Abi quant studio 5 rm 422 machine, by Thermo Fisher Scientific (1 mentions) Sybr select master mix, by Thermo Fisher Scientific (1 mentions) T100 thermal cycler, by Bio-Rad (1 mentions)

Close spelling variants of this product

Reverse transcription kit (1270 citations) RNase inhibitor (943 citations) CDNA transcription kit (24 citations) CDNA kits (3 citations) Transcription Kits (2 citations)

2 protocols using high32capacity cdna reverse transcription kit with rnase inhibitor

1

RNA Extraction and qRT-PCR Analysis

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RNA was extracted from PMN snap frozen pellets with Quick-RNA Microprep or Quick-RNA Miniprep (Zymo Research) per manufacturer’s instructions. cDNA was prepared with High32Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (ThermoFisher Scientific) according to manufacturer’s instructions. qRTPCR were performed in ABI Quant Studio 5 Rm 422 machine (Applied Biosystems). Primer list is provided in Table S1.
Perego M., Fu S., Cao Y., Kossenkov A., Yao M., Bonner E., Alicea-Tores K., Liu W., Jiang Z., Chen Z., Fuchs S.Y., Zhou J, & Gabrilovich D.I. (2022). Mechanisms regulating transitory suppressive activity of neutrophils in newborns: PMN-MDSC in newborns. Journal of leukocyte biology, 112(5), 955-968.
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2

RNA Extraction and Real-Time qPCR

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PANC-1 and CAPAN-2 cells transfected as indicated above were harvested and RNA was immediately extracted using Quick-RNA Microprep (Zymo Research) according to the manufacturer’s instructions. cDNA was prepared with High32 Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (ThermoFisher Scientific) and the reverse-transcription reaction performed on a BioRad T100 Thermal Cycler. Quantitative PCR was performed with SYBR™ Select Master Mix (ThermoFisher) on ABI Quant Studio 5 machine (ThermoFisher).
Kossenkov A.V., Milcarek A., Notta F., Jang G.H., Wilson J.M., Gallinger S., Zhou D.C., Ding L., Ghosh J.C., Perego M., Morotti A., Locatelli M., Robert M.E., Vaira V, & Altieri D.C. (2022). Mitochondrial fitness and cancer risk. PLoS ONE, 17(10), e0273520.
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