Endothelial growth medium egm 2 mv

G-CSF was provided by UBI pharma Inc. (Taipei, Taiwan). Fluorescein isothiocyanate (FITC) anti-mouse Sca-1 and phycoerythrin (PE) anti-mouse Flk-1 antibodies were purchased from eBioscience (San Diego, CA, USA). Isotype-identical antibodies were obtained from Becton Dickinson (Franklin Lakes, NJ, USA). The antibodies listed below were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA): rabbit anti-eNOS, anti-p-eNOS, mouse anti-VEGF, anti-α-SMA, and anti-β-actin. Rabbit antibodies for STAT3 and p-STAT3 were obtained from Cell Signaling Technology (Beverly, MA, USA). Mouse antibodies for IL-10 were purchased from Serotec (Oxford, UK). Rat anti-CD31 was obtained from BD Pharmingen (San Diego, CA, USA). Phenylmethylsulfonyl fluoride (PMSF) protease inhibitor, Histopaque-1077 (density: 1.077 g/mL), and 2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Endothelial growth medium (EGM-2 MV) and growth factors for EPC culture were obtained from Lonza (Morristown, NJ, USA).