Ret 3 iso rectal probe for neonatal rats

A TEMP JKT thermometer (Oakton, Vernon Hills, IL, USA) and an RET-3-ISO rectal probe for neonatal rats (Physitemp, Clifton, NJ, USA) were used to measure the rats’ body temperature, using peanut oil (Acofarma, Terrassa, Spain) for lubrication, as in previous studies [7 (link)]. The initial temperature was evaluated the day before the RV inoculation (the final temperature was determined 1 day after the RV inoculation and the results are expressed as the increase between them). Fecal specimens from rats during the studied period were diluted in distilled water (up to 200 mg/mL), agitated and then the pH was measured using a 5207 pH electrode for surfaces and a micropH 2001 pH meter (Crison Instruments, Barcelona, Spain).

The rats’ body temperature was measured with a TEMP JKT thermometer (Oakton, Vernon Hills, IL, USA) and an RET-3-ISO rectal probe for neonatal rats (Physitemp, Clifton, NJ, USA) and with the aid of peanut oil (Acofarma, Terrassa, Spain) to lubricate. This measure was taken 1–2 days before the inoculation of the virus (initial temperature) and 1–2 days after inoculation of the virus (final temperature). Results were expressed as the absolute increase in temperature taking into account the final temperature (after RV inoculation) with respect to the initial temperature (before RV inoculation).

The pH from fecal diluted samples (up to 200 mg/mL in distilled water) from the peri-inoculation period was measured with a pH electrode for surfaces 5207 and a pH-meter micropH 2001 (Crison Instruments, Barcelona, Spain). Body temperature was measured with the TEMP JKT thermometer (Oakton, Vernon Hills, IL, USA) and the RET-3-ISO rectal probe for neonatal rats (Physitemp, Clifton, NJ, USA). This measure was taken during the peri-inoculation period of the virus. Results were expressed as the relative increase of temperature compared to the temperature the day before the inoculation taken as baseline.