3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt cell viability reagent

Polyethylene glycol (PEG, MW 10,000 Da), ibuprofen sodium, poly(vinyl alcohol) (PVA, MW 31,000–50,000 g/mol), polyvinylpyrrolidone (PVP, MW 40,000 g/mol), alginic acid sodium salt and the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell viability reagent were purchased from Sigma Aldrich, Dorset, UK. Eudragit® S (MW 125,000 g/mol) and Eudragit® L (MW 125,000 g/mol) were obtained from Rohm GmbH & Co.KG, Pharma Polymers, Darmstadt, Germany. Poly(lactic acid) (PLA) was purchased from Futerro, Escanaffles, Belgium. Isocratic HPLC grade methanol and acetonitrile were purchased from VWR International, East Grinstead, UK. L-132 lung epithelial cells were purchased from the American Type Culture Collection (ATCC) and EpiSkin™ was purchased from Skin Ethic Laboratories, Lyon, France. The human IL-1α ELISA kit and Bradford assay kit were purchased from Pierce, Rockford, IL, USA. Gantrez® AN-139, a co-polymer of methyl vinyl ether and maleic anhydride (PMVE/MAH, MW 1,080,000 Da) and Gantrez® MS-955, a mixed sodium and calcium salt of methyl vinyl ether and maleic anhydride copolymer (PVM/MA, MW 1,000,000 Da) were gifts from Ashland, Kidderminster, UK. All other chemicals used were of analytical reagent grade.

Fluorescein isothiocyanate–dextran with an average MW of 70,000 Da (FD 70) and 150,000 Da) were purchased from TdB Consultancy AB (Uppsala, Sweden). Fluorescein sodium (FS) MW 376.27 Da and polyvinylpyrrolidone (PVP K15, MW 10,000 Da; PVP K30, MW 40,000 Da; PVP K90, MW 360,000 Da) were all purchased from Sigma–Aldrich (Dorset, UK). polyvinylpyrrolidone (PVP K29–32, MW 58,000 Da) was a gift from Ashland Inc. (Surrey, UK). ARPE-19 cells were purchased from the American Type Culture Collection. Dulbecco’s modified Eagle’s medium/F-12 human amniotic membrane nutrient mixture (DMEM) was purchased from (Life technologies, UK). Fetal bovine serum (FBS) and trypsin were purchased from Invitrogen Corporation (Paisley, UK). The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell viability reagent was purchased from Sigma Aldrich (Dorset, UK). All other chemicals used were of analytical reagent grade.