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2 protocols using phosphate buffered saline solution

1

Paclitaxel-Coumarin-6 Nanoparticle Evaluation

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Paclitaxel (≥99.0%) and coumarin-6 (≥98.0%) were obtained from Shanghai Aladdin Biochemical Technology, China. Triptolide (≥98.0%) was purchased from Nantong Feiyu Biological Technology, China. Polyvinyl alcohol (PVA, MW 15 000 kDa; hydrolyzed 87%–89%) was purchased from Energy Chemical, China. RIPA lysis buffer and sodium dodecyl sulfate-polyacrylamide gel were obtained from Epizyme, China. Coomassie Brilliant Blue G-250, BCA protein assay kit, Coomassie Blue Staining Solution, antifade mounting medium with DAPI, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were purchased from Beyotime, China. Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich, China. Phosphate buffered saline (PBS) solution was obtained from Cytiva, USA. Transwell plates were obtained from Corning Costar, USA. Crystalline violet staining solution and 4% paraformaldehyde were purchased from Solarbio, China.
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2

SARS-CoV-2 Nucleocapsid Protein Detection

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(3-Aminopropyl)triethoxysilane (APTES, 99%) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). N-Hydroxysuccinimide (NHS), H2O2 (30%), KOH, and glutaraldehyde (GA, 25%) was purchased from Sinopharm Chemical Reagent Co., Ltd. H2SO4 was purchased from Yantai Far East Fine Chemical Co., Ltd (Yantai, China). Phosphate-buffered saline (PBS) solution was purchased from Cytiva. Bovine serum albumin labelled with fluorescein isothiocyanate (BSA-FITC) was purchased from Dalian Meilun Biotechnology Co., Ltd. Glass capillary (0.5 mm in diameter, 8 cm in length) was purchased from the Instrument Plant of West China Medical University (Chengdu, China). Mouse anti-SARS-CoV-2 NP monoclonal antibody-1 (Capture antibody), mouse anti-SARS-CoV-2 NP monoclonal antibody-2 (detection antibody), and recombinant human SARS-CoV-2 NP protein (N-protein) was purchased from Nanjing Baikang Biotechnology Co., Ltd (Nanjing, China). Green fluorescent microspheres was purchased from Huge Biotechnology Co., Ltd (Shanghai, China). MES, Free acid, HEPES was purchased from Coolaber. EDC•HCL was purchased from Solarbio Life Science Co., Ltd (Beijing, China). Green fluorescence microspheres (100 nm) were purchased from Huge Biotechnology. Bovine serum albumin was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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