Frozen muscles were cut at 8–10 microns and sections were air-dried before staining during 30 min. Sections were rehydrated in PBS and incubated for 1 hour with normal goat serum (1:20) followed, only for mice sections, by an 2hours incubation with an anti-mouse IgG unconjugated fab fragment at room temperature. The primary antibodies were left overnight : Dystrophin (1:250, PA1–21011 from Thermo Scientific), beta-dystroglycan (1:400, MANDAG2, from DSHB), nNOS (1:400, sc-462 (R20), SantaCruz), N-terminal dystrophin, Manex1A (1:100, from Dr. G.E. Morris)6 (link). After washes, sections were incubated with the appropriate secondary antibody Alexa Fluor 488 or 568-conjugated for 1h (LifeScience). Slides were covered in Fluoromount plus DAPI (Vector Labs). Observation was realized using Olympus BX61. Acquisitions were taken using DP controller (Olympus).
Sc 462 r20
Manufactured by Santa Cruz Biotechnology
Sc-462 (R20) is a laboratory equipment product offered by Santa Cruz Biotechnology. It serves as a core component for performing various scientific experiments and research activities. The detailed specifications and intended use of this product are not available at this time.
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Lab products found in correlation
2 protocols using sc 462 r20
1
Immunofluorescence Analysis of Dystrophin
2
Immunofluorescence Analysis of Dystrophin
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Frozen muscles were cut at 8–10 microns and sections were air-dried before staining during 30 min. Sections were rehydrated in PBS and incubated for 1 hour with normal goat serum (1:20) followed, only for mice sections, by an 2hours incubation with an anti-mouse IgG unconjugated fab fragment at room temperature. The primary antibodies were left overnight : Dystrophin (1:250, PA1–21011 from Thermo Scientific), beta-dystroglycan (1:400, MANDAG2, from DSHB), nNOS (1:400, sc-462 (R20), SantaCruz), N-terminal dystrophin, Manex1A (1:100, from Dr. G.E. Morris)6 (link). After washes, sections were incubated with the appropriate secondary antibody Alexa Fluor 488 or 568-conjugated for 1h (LifeScience). Slides were covered in Fluoromount plus DAPI (Vector Labs). Observation was realized using Olympus BX61. Acquisitions were taken using DP controller (Olympus).
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