Cells were rinsed twice in ice-cold phosphate-buffered saline (PBS) and fixed with a freshly prepared solution of 4% paraformaldehyde in PBS for 20 minutes and permeabilized for 10 minutes with 0.1% digitonin in PBS. After several rinses in ice-cold PBS, cells were incubated in 3% normal goat serum in PBS (blocking solution) for 30 minutes at room temperature, followed by an overnight incubation at 4°C with primary antibodies. Cells were extensively washed with PBS-0.1% digitonin and then incubated with Alexa-conjugated secondary antibodies (Molecular Probes) for 60 minutes at room temperature. Coverslips were mounted in a mounting medium. Images were captured with an API Delta Vision deconvolution microscope using SoftWorx software (Applied Precision, Issaquah, Washington, USA). Primary antibodies used were anti-AR (Sc-816, 1:200, Santa Cruz Biotechnology Inc.), anti-VAMP7 (NB100-91356, 1:1,000, Novus Biologicals), ESR1 (MA1-310, 1:200, Thermo-Fisher) and anti-RAB5 (#2143, 1:100, Cell Signaling).
Api delta vision
Manufactured by Cytiva
Sourced in United States
The API Delta Vision is a high-performance imaging system designed for advanced microscopy applications. It features a modular design, allowing for customization to meet specific research needs. The system provides precise control and data acquisition capabilities, supporting a range of imaging techniques.
Automatically generated - may contain errors
Lab products found in correlation
Anti vamp7 nb100 91356, by Novus Biologicals (2 mentions)
Esr1 ma1 310, by Thermo Fisher Scientific (2 mentions)
Softworx, by Cytiva (2 mentions)
Anti rab5, by Cell Signaling Technology (2 mentions)
Anti ar sc 816, by Santa Cruz Biotechnology (2 mentions)
Alexa conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)
2 protocols using api delta vision
1
Immunofluorescence Staining of Cellular Proteins
2
Immunofluorescence Staining of Cellular Proteins
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Cells were rinsed twice in ice-cold phosphate-buffered saline (PBS) and fixed with a freshly prepared solution of 4% paraformaldehyde in PBS for 20 minutes and permeabilized for 10 minutes with 0.1% digitonin in PBS. After several rinses in ice-cold PBS, cells were incubated in 3% normal goat serum in PBS (blocking solution) for 30 minutes at room temperature, followed by an overnight incubation at 4°C with primary antibodies. Cells were extensively washed with PBS-0.1% digitonin and then incubated with Alexa-conjugated secondary antibodies (Molecular Probes) for 60 minutes at room temperature. Coverslips were mounted in a mounting medium. Images were captured with an API Delta Vision deconvolution microscope using SoftWorx software (Applied Precision, Issaquah, Washington, USA). Primary antibodies used were anti-AR (Sc-816, 1:200, Santa Cruz Biotechnology Inc.), anti-VAMP7 (NB100-91356, 1:1,000, Novus Biologicals), ESR1 (MA1-310, 1:200, Thermo-Fisher) and anti-RAB5 (#2143, 1:100, Cell Signaling).
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