Axcio observer a1

Immunofluorescence staining of HCV core protein in OR6 cells and JFH1-infected Huh7.5.1 cells were performed as previously described [38 (link)]. OR6, Huh7.5.1 or JFH1 cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% TritonX-100, and blocked with 3% bovine serum albumin in PBS. The primary antibody was mouse anti-HCV core (Thermoscientific). The secondary antibody was goat anti–mouse Alexa Fluor 488 (Invitrogen). DAPI was added to the staining to monitor the nuclear structure. Fluorescence signals were observed by fluorescence microscopy (Zeiss, Axcio Observer A1).

Immunofluorescence staining of HCV core protein in Huh 7.5.1 cells treated with supernatant of JFH-1 infected Huh 7.5.1 cells and Immunofluorescence staining of DENV DV2NS3 protein in Huh 7.5.1 cells treated with supernatant of DENV infected Huh 7.5.1 cells were performed. Huh7.5.1 cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% TritonX-100, and blocked with 3% bovine serum albumin in PBS. The primary antibody was mouse anti-HCV core (Thermoscientific) or anti- DENV DV2NS3 (#YH3304, Yao-Hong Biotechnology, Taipei). The secondary antibody was goat anti–mouse Alexa Fluor 488 (Invitrogen). DAPI was added to the staining to monitor the nuclear structure. Fluorescence signals were observed by fluorescence microscopy (Zeiss, Axcio Observer A1).