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Gfp 2956

Manufactured by Cell Signaling Technology
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GFP (2956) is a recombinant green fluorescent protein (GFP) that can be used as a reporter or tag in various cell and molecular biology applications. It is a 238 amino acid polypeptide derived from the jellyfish Aequorea victoria. GFP (2956) fluoresces green when exposed to blue or ultraviolet light and can be used to monitor gene expression, protein localization, and other cellular processes.

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Lab products found in correlation

Etomoxir, by Merck Group (1 mentions) Menadione, by Merck Group (1 mentions) Oligomycin, by Merck Group (1 mentions) Pf 06424439, by Merck Group (1 mentions) Ebselen, by Bio-Techne (1 mentions) Ab54037, by Abcam (1 mentions) Azd 3988, by Bio-Techne (1 mentions) Tempol, by Merck Group (1 mentions) Paraquat, by Merck Group (1 mentions) Atn 224, by Cayman Chemical (1 mentions) Antibody against erk2 c14, by Santa Cruz Biotechnology (1 mentions) Ml385, by Selleck Chemicals (1 mentions) T5326, by Merck Group (1 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Merck Group (1 mentions) Antimycin a, by Merck Group (1 mentions) Rotenone, by Merck Group (1 mentions) Ferrostatin 1, by Merck Group (1 mentions) Gapdh 60004 1 ig, by Proteintech (1 mentions) Ab46545, by Abcam (1 mentions) N cadherin 13 116, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-GFP antibody (rabbit mAB #2956) Anti-GFP (2956, GFP (D5.1: #2956)

3 protocols using gfp 2956

1

Mitochondrial Stress Response Modulators

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Compounds were used at the following concentrations unless otherwise noted: 50 μM AZD3988 (Tocris), 30 μM A922500 (Stratech), 50 μM AZD7687 (Stratech), 70 μM T863 (Sigma), 1 μM Oligomycin (Sigma), 0.5 μM FCCP (Sigma), 1 μM Antimycin-A (Sigma), 1 μM Rotenone (Sigma), 50 μM PF-06424439 (Sigma), 5 μM Ebselen (Tocris), 1 mM Tempol, 200 μM Paraquat (Sigma), Menadione (Sigma), 100 μM Etomoxir (Sigma), 2 μM Ferrostatin-1 (Sigma), 5 μM ML385 (Selleckchem), and 1 μM ATN-224 (Cayman Chemical). Antibodies against DGAT1 (ab54037) and 4-Hydroxynonenal (ab46545) were purchased from abcam. Antibodies against Vinculin (66305-1-Ig), Beta-Tubulin (10094-1-AP), PINK1 (23274-1-AP), Parkin (14060-1-AP), SOD1 (10269-1-AP), SOD2 (24127-1-AP), SESN2 (10795-1-AP) and GAPDH (60004-1-Ig) were purchased from Proteintech. Antibodies against phospho-AMPK (50081), phospho-RAPTOR (2083), Caspase-3 (9662), phospho-ERK (4370) and GFP (2956) were purchased from Cell Signalling. The antibody against gamma-tubulin (T5326) was purchased from Sigma. The antibody against ERK2 (C-14) was from Santa Cruz Biotechnology.
Wilcock D.J., Badrock A.P., Wong C.W., Owen R., Guerin M., Southam A.D., Johnston H., Telfer B.A., Fullwood P., Watson J., Ferguson H., Ferguson J., Lloyd G.R., Jankevics A., Dunn W.B., Wellbrock C., Lorigan P., Ceol C., Francavilla C., Smith M.P, & Hurlstone A.F. (2022). Oxidative stress from DGAT1 oncoprotein inhibition in melanoma suppresses tumor growth when ROS defenses are also breached. Cell Reports, 39(12), 110995.
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2

Comprehensive Protein Analysis Protocol

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Total protein was extracted with RIPA lysis buffer, containing 25 mM Tris-HCl (pH 7.6), 150 mM NaCl, 1% Nonidet P-40, 1% sodium deoxycholate, 0.1% SDS, complete protease inhibitor, phosphatase inhibitor. Protein lysates were resolved on Bis-Tris gels, transferred to PVDF membranes, probed with primary antibodies overnight at 4 °C and then with HRP-linked secondary antibodies (GE Healthcare, IL) and visualized with ECL reagent (Thermo Fisher Scientific, MA). The following antibodies were used:
AntibodyCat. NoSourceWorking dilution
HA3724Cell Signaling Technology1:2000
Ago22897Cell Signaling Technology1:1000
Caveolin-13267Cell Signaling Technology1:1000
Acetylated lysine9441Cell Signaling Technology1:1000
DDK(Flag)GTX11043GeneTex1:1000
EEA12411Cell Signaling Technology1:1000
CalreticulinGTX111627GeneTex1:1000
HisA00186-100Genescript1:2000
Sirt19475Cell Signaling Technology1:1000
Sirt2GTX129154GeneTex1:1000
HDAC6GTX100722GeneTex1:1000
HDAC106-720Milipore1:1000
GFP2956Cell Signaling Technology1:2000
FibronectinBD610078BD Biosciences1:1000
N-cadherin13116Cell Signaling Technology1:1000
Dicer5362Cell Signaling Technology1:1000
GW182SC374458SantaCruz1:500
GAPDHGTX100118GeneTex1:5000
α-tubulinTA307175Origene1:5000
Integrin β1303010Biolegend1:250
CD81GTX101766GeneTex1:1000
CD63GTX135220GeneTex1:1000
AlixGTX135282GeneTex1:1000
TSG101GTX118736GeneTex1:1000
CD9GTX100912GeneTex1:1000
Apo-A1GTX12692GeneTex1:1000
Lin M.C., Kuo W.H., Chen S.Y., Hsu J.Y., Lu L.Y., Wang C.C., Chen Y.J., Tsai J.S, & Li H.J. (2024). Ago2/CAV1 interaction potentiates metastasis via controlling Ago2 localization and miRNA action. EMBO Reports, 25(5), 2441-2478.
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3

Immunostaining of Skeletal Muscle Samples

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Mouse skeletal muscles were removed as indicated in Results, put in OCT, frozen in dry ice-cooled isopentane (Tissue-Tek), fixed in 4% paraformaldehyde, and blocked in 3% BSA in Tris-buffered saline (TBS). C2C12 cells were fixed in 4% paraformaldehyde and blocked in 3% BSA in PBS. Samples were immunostained overnight with antibodies: AUF1 (07-260, Millipore), Slow myosin (NOQ7.5.4D, Sigma), Fast myosin (MY-32, Sigma), Laminin alpha 2 (4H8-2, Sigma), GFP (2956, Cell Signaling), and Myf5 (clone AF 4027; R&D Systems). Anti myosin type I (BA-D5), IIa (SC-71, and IIb (BF-F3) were from Developmental Studies Hybridoma Bank. Slow and Fast myosin staining was done with the MOM Kit (Vector Labs). Alexa Fluor donkey 488, 555, anti-mouse IgG2b 594 (type I), anti-mouse IgG1 488 (IIa), and anti-mouse IgM 647 (IIb) were from Thermo Fisher Scientific. Secondary antibodies were used at 1:300 and incubated for 1 h at room temperature. Slides were sealed with Vectashield with DAPI (Vector).
Abbadi D., Andrews J.J., Katsara O, & Schneider R.J. (2021). AUF1 gene transfer increases exercise performance and improves skeletal muscle deficit in adult mice. Molecular Therapy. Methods & Clinical Development, 22, 222-236.
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