Dr. S.L. Topalian (National Cancer Institute, USA) provided HLA-A*0201+ 624-MEL cells. The 624-MEL cells were stably transduced to express hPD-L1 and they have been characterized, as previously described [15 (link)]. The 624-MEL cells were cultured in RPMI1640 medium supplemented with 10% Fetal clone I serum (Thermo Scientific, Belgium), 2 mM L-Glutamine, 100 U/mL penicillin, 100 µg/mL streptomycin, 1 mM sodium pyruvate, and nonessential amino acids. Female, five to six weeks old C57BL/6 mice (for biodistribution, biological half-life in blood and stability studies), and athymic nude Crl:NU(NCr)-Foxn1nu mice (for tumor targeting) were purchased from Charles River. All of the experiments were performed in accordance with the European guidelines for animal experimentation under the license LA1230272. Experiments were approved by the Ethical Committee for the use of laboratory animals of the Vrije Universiteit Brussel (17-272-6 and 20-272-4). Intravenous injections were performed in the tail vein. The animals were anesthetized with 2.5% isoflurane in oxygen (Abbott) for injections, samplings, imaging, and euthanasia.
Athymic nude crl nu ncr foxn1nu mice
Manufactured by Charles River Laboratories
Sourced in Canada
Athymic nude Crl:NU(NCr)-Foxn1nu mice are an immunodeficient mouse model that lacks functional T cells. These mice are commonly used in biomedical research to study tumor growth, engraftment of human cells and tissues, and the evaluation of therapeutic interventions.
Automatically generated - may contain errors
Lab products found in correlation
Fetal clone 1 serum, by Thermo Fisher Scientific (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
Sprague dawley rat, by Charles River Laboratories (1 mentions)
H1975, by American Type Culture Collection (1 mentions)
Balb cj mice, by Jackson ImmunoResearch (1 mentions)
Skh 1 elite mice crl skh1 hrhr, by Charles River Laboratories (1 mentions)
5 protocols using athymic nude crl nu ncr foxn1nu mice
1
Characterization of Transgenic Murine Melanoma Model
2
In Vivo Mouse Cancer Studies
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All animal studies were conducted with approval by the Saint Louis University Institutional Animal Care Committee (IACUC; protocol #2368) and all experiments were carried out with strict accordance with relevant guidelines and regulations. Six-week-old C57BL/6J mice were obtained from Jackson Laboratories. Athymic nude [Crl:NU(NCr)-Foxn1nu] mice were obtained from Charles River Laboratories. Mice were housed in sterile ventilated cages under a standard dark light cycle. They were provided a standard chow diet and water ad libitum.
3
Therapeutic Evaluation in Athymic Mice
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All experiments were approved by the Animal Care Committee at the Research Institute of the McGill University Health Centre (RI-MUHC) and in accordance with the ethical guidelines of the Canadian Council on Animal Care (Protocol Number: 8117). Athymic Nude Crl:NU(NCr)-Foxn1nu mice (Charles River Laboratories, Canada) used for experiments were housed at the Animal Resources Division of the RI-MUHC. Animals were housed maximum of 5 per cage and fed water and standard mice kibble ad libitum. All surgeries were performed with animals anesthetized with 2% isoflurane gas and with subcutaneous injection of slow-release buprenorphine as an analgesic. Animals were euthanized at 30 days post-treatment administration or if the animals reached the humane endpoint. Euthanization was conducted using 5% isoflurane anesthetization, followed by carbon dioxide asphyxiation and cervical dislocation. All efforts were made to minimize the suffering of the animals.
4
Topotecan Formulation and Preclinical Evaluation
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The spray-dried powder formulation of topotecan was manufactured from (S)-topotecan (Toronto Research Chemicals, Inc., Toronto, Canada) and its physical, chemical, and aerosol characteristics were determined as described (Kuehl et al., 2018 (link)). The aqueous formulation of topotecan for IV delivery was prepared immediately prior to injection according to the recommendation for HYCAMTIN® (topotecan) for injection. A total of 48 male 6–8 weeks old Rowett nude rats (Cr:NIH-rnu) for the orthotopic lung cancer study were obtained from Envigo (Indianapolis, IN). Sixty female, 6–8 weeks old, CD-1® IGS mice for pharmacokinetics (PKs), 54 female, 6–8 weeks old athymic nude mice (Crl:NU(NCr)-Foxn1nu) for subcutaneous xenografts, and 12 male, 8 weeks old Sprague Dawley rats for a pilot cumulative toxicity study were all obtained from Charles River Laboratories. Authenticated human lung adenocarcinoma cells lines (A549, H358, and H1975) were obtained from American Type Culture Collection (ATCC), maintained according to ATCC protocols, and used within 6 months post-resuscitation. All animal studies were conducted at Lovelace Biomedical under protocols approved by the Lovelace Institutional Animal Care and Use Committee and facilities that are accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International.
5
Murine Models for Skin Cancer Research
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All animal studies were carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals (81 ) and with the prior approval of the Animal Care and Use Committee of The Johns Hopkins University (MO12M223). Four- to five-week-old male and female SKH-1 Elite mice (Crl:SKH1-Hrhr) and athymic nude mice [Crl:NU(NCr)-Foxn1nu] were obtained from Charles River Inc. BALB/cJ mice (strain code 000651) were obtained from The Jackson Laboratory.
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