Photomicrographs were obtained with an Axiolab microscope (Carl Zeiss Microscopy) and a SZX12-ILLB2-200 binocular microscope (Olympus). Fluorescence microscopy was performed with a Zeiss AxioObserver Z.1 inverted confocal microscope, equipped with Plan-Neofluar 63x/0.75 (air) and Plan-Apochromat 100x/1.4 oil objectives (Zeiss). The SlideBook 6.0 software (Intelligent Imaging Innovations) was used for picture processing.
Strains were grown in 8-well borosilicate cover glass system (Thermo Scientific) in 400 μl MM supplemented as mentioned above, when needed, or on glass slides covered with 1 ml solid MM supplemented as mentioned above, when needed, at 37°C or 30°C. GFP-signals were normalized against wildtype background signal to subtract fungal auto fluorescence. Nuclei were visualized by ectopic integration of pgpdA::rfp::h2A into the respective strains or through staining with 0.1% 4’,6’-diamidino-2phenylindole (DAPI).
Strains were grown in 8-well borosilicate cover glass system (Thermo Scientific) in 400 μl MM supplemented as mentioned above, when needed, or on glass slides covered with 1 ml solid MM supplemented as mentioned above, when needed, at 37°C or 30°C. GFP-signals were normalized against wildtype background signal to subtract fungal auto fluorescence. Nuclei were visualized by ectopic integration of pgpdA::rfp::h2A into the respective strains or through staining with 0.1% 4’,6’-diamidino-2phenylindole (DAPI).