Cells were seeded in 384-well plates (ViewPlate-384 Black - PerkinElmer, ref. 6007460) using a MultiDrop combi (ThermoFisher Scientific), in 40 μL of cell media at 37 °C for 24 h. Optimal cell densities were determined as 5 × 103 for CTR and NF1KO_1 and 6 × 103 for NF1KO_2 TF-1 cell lines using T4 Cellometer (Nexcelom). Detailed procedures and data processing methods are provided in the Supplementary Material section.
Viewplate 384 black
Manufactured by PerkinElmer
The ViewPlate-384 Black is a 384-well microplate designed for cell-based assays and screening applications. It features a black polystyrene well plate with a clear bottom, providing optical clarity for imaging and detection. The plate is suitable for a variety of cell-based assays and high-throughput screening experiments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using viewplate 384 black
1
Cell Seeding and Density Optimization for TF-1 Cells
2
Culturing Malignant Pleural Mesothelioma Cell Lines
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Patient-derived malignant pleural mesothelioma cell lines MPM04/BAR, MPM10/CORO, MPM11/DEL, MPM17/ GAG, MPM24/MART, MPM25/MAY, MPM28/MLD, MPM59/LLA, and MPM60/MASS cells (obtained from Didier Jean, INSERM UMR 1162, Paris, France) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco Life Technologies, Waltham, MA), supplemented with 10% (v/v) fetal bovine serum (20% for MPM11/DEL) (Gibco Life Technologies) and 1% (v/v) penicillin/streptomycin (Gibco Life Technologies) in a 5% CO 2 humidified atmosphere at 37 °C. The prostate IGR-Cap1 R100 cell line was grown as described previously. 22 The medium was replaced every 3-4 days.
For compound screening, cells were counted with a T4 Cellometer cell counter (Nexcelom, Lawrence, MA) and then seeded in 384-well plates (ViewPlate-384 Black, PerkinElmer, Waltham, MA) in 40 µL of cell media using the MultiDrop Combi (Thermo Fisher Scientific, Waltham, MA). Cell line densities were empirically determined: 3000 cells per well for IGR-Cap-100; 1750 cells per well for MPM10/CORO, MPM17/GAG, and MPM24/MART; 2000 cells per well for MPM04/BAR, MPM25/MAY, and MPM59/LLA; 2500 cells per well for MPM60/MASS; and 3000 cells per well for MPM11/DEL and MPM28/MLD. The screen was performed at early cell passages for all replicates after the cells had been thawed from liquid nitrogen and passaged three times.
For compound screening, cells were counted with a T4 Cellometer cell counter (Nexcelom, Lawrence, MA) and then seeded in 384-well plates (ViewPlate-384 Black, PerkinElmer, Waltham, MA) in 40 µL of cell media using the MultiDrop Combi (Thermo Fisher Scientific, Waltham, MA). Cell line densities were empirically determined: 3000 cells per well for IGR-Cap-100; 1750 cells per well for MPM10/CORO, MPM17/GAG, and MPM24/MART; 2000 cells per well for MPM04/BAR, MPM25/MAY, and MPM59/LLA; 2500 cells per well for MPM60/MASS; and 3000 cells per well for MPM11/DEL and MPM28/MLD. The screen was performed at early cell passages for all replicates after the cells had been thawed from liquid nitrogen and passaged three times.
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