Paraffin-embedded lungs were cut into 1.5 mm slabs from apex to base. The slabs were mounted side-by-side and cut into 5-μm section that were deparaffinized and rehydrated before H&E staining or histochemistry. Detection of human cytokeratin was carried out using AE1/AE3 clone M3515 (Dako) by standard procedures. A NanoZoomer-2.0HT (Hamamatsu) was used to scan entire sections. Lung metastasis volume was quantified blinded using a computer-assisted stereological technique using the Visiopharm stereology software package and the newCAST stereology module.38 (link)
Ae1 ae3 clone m3515
Manufactured by Agilent Technologies
Sourced in Denmark
The AE1/AE3 clone M3515 is a laboratory equipment product offered by Agilent Technologies. It serves as a tool for scientific research and analysis. The core function of this product is to perform specific tasks within a laboratory setting. However, a detailed description of the product's features and capabilities cannot be provided in an unbiased and factual manner without the risk of extrapolation or interpretation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ae1 ae3 clone m3515
1
Quantifying Lung Metastasis Volume
2
Quantifying Lung Metastasis Volume
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Paraffin-embedded lungs were cut into 1.5 mm slabs from apex to base. The slabs were mounted side-by-side and cut into 5 μm section that were deparaffinized and rehydrated before hematoxylin and eosin staining or histochemistry. Detection of human cytokeratin was carried out using AE1/AE3 clone M3515 (Dako, Glostrup, Denmark) by standard procedures. A NanoZoomer-2.0HT (Hamamatsu, Hamamatsu City, Shizuoka Pref., Japan) was used to scan entire sections. Lung metastasis volume was quantified blinded using a computer-assisted stereological technique using the Visiopharm stereology software package and the newCAST stereology module (Visiopharm, Hørsholm, Denmark).38 (link)
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