Images of retinas were taken using a LSM 780 inverted microscope (Zeiss) equipped with a Plan-Apochromat 20×/0.8 NA Ph2 objective or with a Plan-Apochromat 63×/1.4 NA DIC objective or using a LSM 700 upright microscope (Zeiss) equipped with a Plan-Apochromat 20×/0.8 NA Ph2 objective. Both microscopes were equipped with a photon multiplier tube detector. Images were taken at room temperature using Zen 2.3 software (Zeiss).
Plan apochromat 20 0.8 na ph2 objective
Manufactured by Zeiss
The Plan-Apochromat 20x/0.8 NA Ph2 objective is a high-performance microscope objective from Zeiss. It is designed to provide a wide field of view with excellent resolution, color correction, and contrast. The objective has a numerical aperture of 0.8 and is compatible with phase contrast imaging techniques.
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Lab products found in correlation
2 protocols using plan apochromat 20 0.8 na ph2 objective
1
Retinal Imaging with Confocal Microscopy
2
Live-cell Imaging of Arl13b2-mEGFP in HUVEC
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HUVEC cells were plated in ibidi slides 0.6 and infected with the Arl13b2-mEGP virus. 48 h later, cells were stained with 1 µM siR-DNA nuclear dye (Spirochrome) and incubated for an additional hour. Cells were subsequently imaged using an LSM 780 inverted microscope (Zeiss) equipped with Plan-Apochromat 20×/0.8 NA Ph2 objective and the Definite Focus system. Time series of single planes were acquired at five randomly selected positions with 5 min resolution, 0.42 × 0.42 µm pixel, and an open pinhole. Images were taken at 37°C, 5% CO2 using Zen 2.3 software. Images were analyzed in Fiji (Schindelin et al., 2012 (link)).
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