Mouse anti foxj1

Spinal cord and sciatic nerves were harvested as for RT-PCR. Protein extraction was performed using CelLytic MT Cell Lysis buffer (Sigma-Aldrich) supplemented with protease inhibitor mixture. Equal amounts of protein were denatured in sample buffer and resolved on 4–12% SDS-polyacrylamide gels (Invitrogen). Foxj1 was detected using mouse anti-foxj1 (Thermo Fisher Scientific) and visualized with ECL Plus (GE Healthcare).

The following antibodies were used: rat anti-CDH1 (1:200, Santa Cruz, sc-59778); mouse anti-CDH1 (1:100, BD Biosciences, 560062); rabbit anti cleaved caspase-3 (1:600, Cell Signaling Technologies, #9661); goat anti-SCGB1A1 (1:200, Santa Cruz, T-18); rabbit anti-NKX2.1 (1:400, Santa Cruz, H-190); mouse anti-acetyl-α tubulin (1:2000, Sigma, MABT868); mouse anti-FOXJ1 (1:400, Thermo Fisher Scientific, 14-9965-82); rabbit anti-MUC5AC (1:400, Santa Cruz, H-160); rabbit anti-MUC5B (1:400, Novus Biologicals, NBP1-92151); mouse anti-BrdU (1:400, Thermo Fisher Scientific, B35141); hamster anti-PDPN (1:20, DSHB, 8.1.1); rat anti-SCGB3A1 (1:200, R&D Systems, MAB2954); rat anti-SCGB3A2 (1:200, R&D Systems, MAB3465); rabbit anti-Ki67 (1:400, Thermo Fisher Scientific, PA5-19462); goat anti-NGFR (1:200, Santa Cruz, C-20); mouse anti-α-SMA-Cy3 (1:1000, Sigma-Aldrich, C6198); rabbit anti-SOX9 (1:400, Millipore, AB5535MA); goat anti-SOX9 (1:500, R&D Systems, AF3075); rat anti-Cd140a (1:100, Biolegend, 135901); rabbit anti-RFX3 (1:500, Sigma, HPA035689); mouse anti-TRP63 (1:500, Abcam, ab735); rabbit anti-TRP63 (1:100, Cell Signaling Technology, 13109S) and chicken anti-KRT5 (1:400, Biosite, 905901).

The primary antibodies used were as follows: mouse anti-β-catenin (BD Biosciences, 610153), rabbit anti-β-catenin (Sigma-Aldrich, C2206), rabbit anti-C11orf95/ZFTA (Abcam, ab170283), rat anti-CD24 conjugated with FITC (Thermo Fisher, 11-0242-82), rabbit anti-Doublecorin (DCX, cell signalling, #4604), mouse anti-FoxJ1 (eBiosciences, 14-9965), mouse anti-Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Millipore, MAB374), chicken anti-GFAP (Aves, GFAP), rabbit anti-RFX1 (Novus, NBP1-52654), rabbit anti-S100β (Proteintech, 15146-1-AP), rabbit anti-smooth muscle actin (αSMA, Proteintech, 55135-1-AP), mouse anti-acetylated tubulin (Sigma-Aldrich, T6793) and rabbit anti-γ-tubulin (Sigma-Aldrich, T5192). The secondary antibodies used were as follows: conjugated to Alexa Fluor dyes (Abcam) or HRP (Jackson ImmunoResearch).