PCR amplification of DNA was performed using a LifeECO thermal cycler (version 1.04; Bioer Technology) and Quick Taq HS DyeMix DNA polymerase (Toyobo). Sanger sequencing was entrusted to Fasmac. PCR products were purified using a high pure PCR product purification kit (Roche). Customized oligonucleotide primers (see Table S1 in the supplemental material) were purchased from Fasmac and Hokkaido System Science. Chromosomal and plasmid DNA molecules were extracted with a DNeasy blood and tissue kit (Qiagen) and a high pure plasmid isolation kit (Roche), respectively.
Quick taq hs dyemix dna polymerase
Manufactured by Toyobo
Quick Taq HS DyeMix DNA polymerase is a thermostable DNA polymerase designed for rapid and efficient DNA amplification. It is a ready-to-use solution that combines the polymerase enzyme with the necessary reagents, including dNTPs and a proprietary dye mix, in a single tube.
Automatically generated - may contain errors
2 protocols using quick taq hs dyemix dna polymerase
1
Molecular Cloning and Sequencing Protocol
2
PCR and Sanger Sequencing of DNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PCR amplification of DNA was performed using a LifeECO thermal cycler (version 1.04, Bioer Technology) and Quick Taq® HS DyeMix DNA polymerase (Toyobo).
Sanger sequencing was performed by Fasmac. PCR products were purified using a High Pure PCR Product Purification Kit (Roche). Customized oligonucleotide primers (S1 Table ) were purchased from Fasmac and Hokkaido System Science. Chromosomal and plasmid DNA molecules were extracted with a DNeasy Blood and Tissue Kit (Qiagen) and a High Pure Plasmid Isolation Kit (Roche), respectively.
Sanger sequencing was performed by Fasmac. PCR products were purified using a High Pure PCR Product Purification Kit (Roche). Customized oligonucleotide primers (S1 Table ) were purchased from Fasmac and Hokkaido System Science. Chromosomal and plasmid DNA molecules were extracted with a DNeasy Blood and Tissue Kit (Qiagen) and a High Pure Plasmid Isolation Kit (Roche), respectively.
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