Male DBA/1 mice were immunized with collagen and CFA and divided into two groups. One group received neutralizing antibody to IL-22 and another group received mouse IgG isotype control antibody. Mice were scored for arthritis every other day. Anti-IL-22 antibody (clone 8E11, a kind gift from Dr. Wenjun Ouyang, Genentech, San Francisco, CA) or mouse IgG isotype control antibody (Biolegend) were administered intraperitoneally at 100 ug/day/mouse for 8–10 days, starting day 18 post immunization.
Mouse igg isotype control antibody
Manufactured by BioLegend
The Mouse IgG isotype control antibody is a laboratory reagent used to establish background staining levels in flow cytometry and other immunoassays. It is an immunoglobulin G (IgG) antibody that does not bind to any known antigen, serving as a non-specific control for experiments involving mouse antibodies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse igg isotype control antibody
1
Collagen-induced Arthritis Modulation
2
Quantifying GH Expression via Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate the level of GH expression, cells were suspended and incubated with the anti-GH antibody (clone VK9; Thermo Fisher) or mouse IgG isotype control antibody (BioLegend) at 4 C for 1 hour. After being washed once with PBS, cells were then incubated with goat anti-mouse IgG-PE (BioLegend) followed by analysis with flow cytometry. Because anti-CD30 ADC was used as a control ADC (Ctrl-ADC) in this study, the level of CD30 expression was also evaluated using an anti-CD30-FITC antibody (BioLegend). Each cell line used in the study showed negative CD30 expression.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!