EMSAs were performed as previously described (Zhang et al., 2021) . Brie y, MBP-MYB75 was expressed in E. coli and puri ed using MBP Sepharose beads, respectively. The DNA fragment (Supplementary Table S1) was incubated with MBP-MYB75 in EMSA binding buffer (25 mM HEPES-KOH, pH 8.0, 50 mM KCl, 1 mM dithiothreitol [DTT] and 10% glycerol). An Electrophoretic Mobility Shift Assay (EMSA) Kit with SYBR Green and SYPRO Ruby EMSA stains (Thermo Fisher) were used to detect protein-DNA interactions.
Sypro ruby emsa stains
Manufactured by Thermo Fisher Scientific
SYPRO Ruby EMSA stains are fluorescent dyes used for the detection and visualization of proteins in electrophoretic mobility shift assays (EMSAs). The dyes specifically bind to proteins and emit a fluorescent signal, allowing for the identification and analysis of protein-DNA or protein-protein interactions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sypro ruby emsa stains
1
Protein-DNA Interaction Analysis via EMSA
2
Protein-DNA Interaction Analysis via EMSA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EMSAs were performed as previously described (Zhang et al., 2021) . Brie y, MBP-MYB75 was expressed in E. coli and puri ed using MBP Sepharose beads, respectively. The DNA fragment (Supplementary Table S1) was incubated with MBP-MYB75 in EMSA binding buffer (25 mM HEPES-KOH, pH 8.0, 50 mM KCl, 1 mM dithiothreitol [DTT] and 10% glycerol). An Electrophoretic Mobility Shift Assay (EMSA) Kit with SYBR Green and SYPRO Ruby EMSA stains (Thermo Fisher) were used to detect protein-DNA interactions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!