The automated analyzer was used for measurements of CBC (Mindray BC-5000VET, Shenzhen
Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China) and serum chemistry profiles
(BUN, Cr, ALT, TP and albumin) (ILAB 650 Chemistry Analyzer, Diamond diagnostics,
Holliston, MA, USA). The concentration of serum SDMA was measured by commercial analyzer
(Vcheck V200, Bionote Co., Ltd., Gyeonggi-do, South Korea). The information from
manufacturer showed the close relationship between concentration of SDMA obtained from
this kit and from gold standard “I” laboratory (R2=0.9908). This method had the
lowest detecting value of 10 µg/dl and highest detecting value of 100 µg/dl. For iron
parameters, serum iron concentration and unsaturated iron binding capacity (UIBC) were
analyzed by the standard colorimetric Ferrozine method (Cobas c501, Roche Diagnostics,
Indianapolis, IN, USA). The TIBC was calculated as TIBC=UIBC + serum iron concentration.
Serum hepcidin levels were measured by Sandwich quantitative ELISA technique using canine
hepcidin (HEPC) ELISA kit (Catalogue # MBS010437; MyBiosource Inc., San Diego, CA, USA).
The percent saturation (%Sat) was calculated as the ratio of serum iron concentration to
TIBC.
Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China) and serum chemistry profiles
(BUN, Cr, ALT, TP and albumin) (ILAB 650 Chemistry Analyzer, Diamond diagnostics,
Holliston, MA, USA). The concentration of serum SDMA was measured by commercial analyzer
(Vcheck V200, Bionote Co., Ltd., Gyeonggi-do, South Korea). The information from
manufacturer showed the close relationship between concentration of SDMA obtained from
this kit and from gold standard “I” laboratory (R2=0.9908). This method had the
lowest detecting value of 10 µg/dl and highest detecting value of 100 µg/dl. For iron
parameters, serum iron concentration and unsaturated iron binding capacity (UIBC) were
analyzed by the standard colorimetric Ferrozine method (Cobas c501, Roche Diagnostics,
Indianapolis, IN, USA). The TIBC was calculated as TIBC=UIBC + serum iron concentration.
Serum hepcidin levels were measured by Sandwich quantitative ELISA technique using canine
hepcidin (HEPC) ELISA kit (Catalogue # MBS010437; MyBiosource Inc., San Diego, CA, USA).
The percent saturation (%Sat) was calculated as the ratio of serum iron concentration to
TIBC.