L α phosphatidylcholine

Carvedilol was purchased from TCI America (Portland, OR, USA). Tween-80 and sodium cholate were purchased from VWR (Radnor, PA, USA). l-α-phosphatidylcholine (Soy PC or SPC), l-α-phosphatidylcholine, hydrogenated (Hydro Egg PC or HEPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA).

Perfluorooctylbromide (PFOB), 1H,1H,2H,2H-perfluorode-canethiol (PFDT), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and cholesterol were supplied by Sigma-Aldrich (St. Louis, MO). Perfluoro-15-crown ether (PFCE) was purchased from SynQuest Laboratories (Alachua, FL). 3-(N-succinimidyloxyglutaryl) aminopropyl polyethyleneglycol-carbamyldistearoyl phosphatidyl-ethanolamine (DSPE-PEG₃₄₀₀-NHS) was supplied by NOF Co. (Tokyo, Japan) and CdSe/ZnS QDs was obtained from Evident Technologies (Troy, NY). L-α-phosphatidylcholine (lecithin, 95%, chicken egg) was purchased from Avanti Polar Lipids (Alabaster, AL). Antibodies were from following sources: anti-human EGF1R (AbD serotec, Oxford, UK), anti-human ErbB2 and anti-human IGF1R (R&D Systems, Inc., Minneapolis, MN). All compounds for the cell culture were supplied by Invitrogen (Carlsbad, CA).

Erufosine (EPC3) was synthesized in the Max Planck Institute for Biophysical Chemistry, Göttingen, Germany, and was most graciously provided by Prof. Martin R. Berger. It was dissolved in a PBS (phosphate buffer saline, 137 mM NaCl, 2.7 mM KCl, 8 mM Na₂HPO₄, and 2 mM KH₂PO₄, pH 7.4) in 10 mM stock solution and kept at 4 °C. Di-4-ANEPPDHQ and Laurdan (6-dodecanyl-2-dimethylaminonaphtalene) were from Molecular Probes (Eugene, OR, USA). l-α-phosphatidylcholine from chicken egg (eggPC), cholesterol from ovine wool (Chol), sphingomyelin from porcine brain (bSM), 23-(dipyrrometheneboron difluoride)-24-norcholesterol (TF-Chol) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-n-(lissamine rhodamine B sulfonyl) (Rhod–DOPE) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA).

l-α-Phosphatidylcholine (PC,
>95%, from soy) was purchased from Avanti Polar Lipids. Fluorescein
isothiocyanate–dextran (FITC–dextran, M_w 3–5 kDa) was purchased from Sigma-Aldrich. All
other chemicals and solvents were purchased from Sigma-Aldrich and
used as received unless otherwise noted. The solvents used for polymerization
were further purified by using alumina columns under argon protection.
CD₂Cl₂ and CDCl₃ were purchased from
Cambridge Isotope laboratories. ¹H NMR was collected by
Bruker AV-400 III spectrometer at 298 K and analyzed by using Topspin
software. Chemical shifts (δ) given in parts per million (ppm)
were referenced to protio impurities.

The following materials were obtained from the sources shown in parentheses: thromboplastin (TF), kaolin (Renam, Moscow, Russia), fVIIa (Novo Nordisk, Bagsvaerd, Denmark) fVa, fIXa, fXIa, fV, fIX, Anti-Human Factor XI antibody (Haematologic Technologies Inc., Vermont, USA), fII (Enzyme Research Laboratories Inc, South Bend, IN, USA), fVIII (Hemophil M, Baxter, Moscow, Russia), L-α-phosphatidylserine (Brain, Porcine) (PS), L-α-phosphatidylcholine (Egg, Chicken) (PC) (Avanti Polar Lipids, Alabaster, Alabama, USA), specific fXIIa inhibitor corn trypsin inhibitor (CTI) (Institute of Protein Research, Russian Academy of Sciences, Pushchino, Russia), D-phenylalanyl-L-prolyl-L-arginine chloromethyl ketone (PPACK), (Calbiochem, Emd Biosciences Inc, San Diego, CA, USA), mouse anti-human CD61 labeled with peridinin-chlorophyll-protein complex (anti-CD61-PerCP), annexin V-FITC (BD Biosciences, San Jose, CA, USA), chromogenic substrate for fXIIa, fXIa and kallikrein S2302 (CHROMOGENIX, Instrumentation Laboratory company, Bedford, MA, USA), fluorogenic substrate for thrombin Z-Gly-Gly-Arg-AMC·HCI (Bachem Americas, Inc., Torrance, CA, USA). Nitrophorin 2 was prepared as described [16] . All other reagents were obtained from Sigma-Aldrich (St. Louis, Missouri, USA).