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Standard fmoc protected amino acids

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Standard Fmoc-protected amino acids are a common set of chemical reagents used in the synthesis of peptides and proteins. They provide the building blocks for constructing the desired amino acid sequence. These amino acids are protected with the Fmoc (fluorenylmethyloxycarbonyl) group, which allows for controlled, step-wise addition during peptide synthesis.

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Lab products found in correlation

4800 maldi tof tof mass spectrometry, by AB Sciex (2 mentions) High performance liquid chromatography, by Thermo Fisher Scientific (2 mentions) Hplc grade solvents, by Thermo Fisher Scientific (2 mentions) Agilent 1260 series hplc system, by Agilent Technologies (2 mentions) Acetyl coenzyme a lithium salt, by Merck Group (2 mentions)

2 protocols using standard fmoc protected amino acids

1

Peptide Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The standard Fmoc-protected amino acids were purchased from CEM Corporation. Acetyl coenzyme A lithium salt was purchased from Sigma-Aldrich. ThioGlo4 was purchased from Berry & Associates. High-performance liquid chromatography (HPLC) grade solvents were purchased from Fisher Scientific. Matrix-assisted laser desorption/ionization (MALDI) spectra were acquired using positive-ion mode on a 4800 MALDI TOF/TOF mass spectrometry (Sciex). Peptides were synthesized on a CEM Liberty Blue peptide synthesizer. The purity of the peptides was confirmed by Agilent 1260 Series HPLC system, eluting with a 0-40% acetonitrile/water gradient with 0.1% (v/v) TFA. All the purity of final peptides showed >95%. The spectra of MALDI and HPLC are available in the Supporting information (Figure S3).
Ho Y.H, & Huang R. (2023). Effects of Oncohistone Mutations and PTM Crosstalk on the N-Terminal Acetylation Activities of NatD. ACS chemical biology, 18(4).
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2

Synthesis and Characterization of Peptides

Check if the same lab product or an alternative is used in the 5 most similar protocols
The standard Fmoc-protected amino acids were purchased from CEM Corporation. Acetyl coenzyme A lithium salt was purchased from Sigma-Aldrich. ThioGlo4 was purchased from Berry & Associates. High-performance liquid chromatography (HPLC) grade solvents were purchased from Fisher Scientific. Matrix-assisted laser desorption/ionization (MALDI) spectra were acquired using positive-ion mode on a 4800 MALDI TOF/TOF mass spectrometry (Sciex). Peptides were synthesized on a CEM Liberty Blue peptide synthesizer. The purity of the peptides was confirmed by Agilent 1260 Series HPLC system, eluting with a 0–40% acetonitrile/water gradient with 0.1% (v/v) TFA. All the purity of final peptides showed >95%. The spectra of MALDI and HPLC are available in the Supporting information (Figure S3).
Ho Y.H, & Huang R. (2022). Effects of Oncohistone Mutations and PTM Crosstalk on the N-terminal Acetylation Activities of NatD. ACS chemical biology, 18(4), 693-700.
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