Four hundred rat IEQs were lysed in RIPA buffer (Sigma-Aldrich, St. Louis, MO, USA) supplemented with protease inhibitor cocktails (Roche, Basel, Switzerland). Proteins were separated by SDS-10% PAGE (Biorad), transferred to nitrocellulose membranes and incubated overnight with the following primary antibodies: anti-superoxide dismutase 1 (SOD1) (1:2000), anti-NAD(P)H dehydrogenase (quinone 1) (NQO1) (1:1000), anti-heme oxygenase 1 (HO-1) (1:1000) and anti-ferritin H (FTH) (1:1000) (Abcam, Cambridge, UK). The signals were detected with horseradish peroxidase-conjugated secondary antibodies: goat anti-rabbit IgG (1:10,000) and goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA), and revealed with an enhanced chemiluminescence system (Thermo Fisher). Due to our experimental conditions mimicking inflammation, we were unable to find a stable protein as a loading control. To address this issue, blot signals were normalized with Ponceau S staining of each lane.
Anti nad p h dehydrogenase quinone 1 nqo1
Manufactured by Abcam
Sourced in United Kingdom, United States
Anti-NAD(P)H dehydrogenase (quinone 1) (NQO1) is a protein that catalyzes the reduction of quinones and other compounds. It plays a role in the detoxification of environmental pollutants and the activation of some anticancer drugs.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Goat anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Enhanced chemiluminescence system, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Anti superoxide dismutase 1 sod1, by Abcam (1 mentions)
Anti heme oxygenase 1 ho 1, by Abcam (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Proteintech (1 mentions)
Anti 3 nitrotyrosine 3 nt, by Merck Group (1 mentions)
Anti txnip, by Abcam (1 mentions)
Anti asc, by Abcam (1 mentions)
Anti nrf2, by Abcam (1 mentions)
Anti caspase 1, by Abcam (1 mentions)
Anti nlrp3, by Abcam (1 mentions)
Anti il 1β, by Abcam (1 mentions)
Anti il 18, by Abcam (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
2 protocols using anti nad p h dehydrogenase quinone 1 nqo1
1
Western Blot Analysis of Antioxidant Proteins
2
Antibody Profiling for Oxidative Stress
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The primary antibodies used for western blot assay and immunohistochemical analysis were as follows:
anti-GAPDH (Proteintech, Wuhan, China); anti-Catalase (CAT), anti-SOD2 (Cell Signaling Technology, Danvers, MA, USA); anti-3-Nitrotyrosine (3-NT, Millipore); anti-NAD(P)H dehydrogenase quinone 1 (NQO-1), anti-Nrf2, anti-TXNIP, anti-NLRP3, anti-Caspase-1, anti-IL-18, anti-IL-1β, and anti-ASC (Abcam, Cambridge, UK). Horseradish peroxidase (HRP)-conjugated secondary antibodies used for western blot analysis were purchased from Proteintech (Wuhan, China). The SPlink Detection Kits used for immunohistochemical analysis were purchased from ZSGB-BIO Technology (Beijing, China).
anti-GAPDH (Proteintech, Wuhan, China); anti-Catalase (CAT), anti-SOD2 (Cell Signaling Technology, Danvers, MA, USA); anti-3-Nitrotyrosine (3-NT, Millipore); anti-NAD(P)H dehydrogenase quinone 1 (NQO-1), anti-Nrf2, anti-TXNIP, anti-NLRP3, anti-Caspase-1, anti-IL-18, anti-IL-1β, and anti-ASC (Abcam, Cambridge, UK). Horseradish peroxidase (HRP)-conjugated secondary antibodies used for western blot analysis were purchased from Proteintech (Wuhan, China). The SPlink Detection Kits used for immunohistochemical analysis were purchased from ZSGB-BIO Technology (Beijing, China).
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