Doxycycline diet

Manufactured by Inotiv

Sourced in United States

Doxycycline diet is a lab equipment product used to administer doxycycline, a tetracycline antibiotic, to laboratory animals as part of research protocols.

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Lab products found in correlation

Foxn1nu nu, by Inotiv (1 mentions) Female nod scid mice, by Jackson ImmunoResearch (1 mentions) H 2kd, by BD (1 mentions) Matrigel, by Corning (1 mentions) Doxycycline hyclate, by Merck Group (1 mentions)

Close spelling variants of this product

Doxycycline (38 citations) Doxycycline rodent diet (3 citations)

6 protocols using doxycycline diet

Genetically Engineered Mouse Models for Lung Cancer

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Rb1^lox/lox; Trp53^lox/lox (Rb1/Trp53-GEMM) and Rb1^lox/lox; Trp53^lox/lox; Rbl2^lox/lox (Rb1/Trp53/Rbl2-GEMM) have been previously described (14 (link), 16 (link)). Mouse strains with Fgfr1^lox and Fgfr2^lox alleles have been previously described (17 (link), 18 (link)). For tumor induction, lungs of 10-week-old mice, including both male and female mice and littermates, were infected with adenoviral Cre via intratracheal instillation as previously described and mice were aged 6 months (19 (link)). For allograft experiment, we inject 5.0×10⁵ control or Fgfr1-preSC in the flanks of nude mice (Foxn1^nu/nu; Envigo) and 1.0×10⁶Fgfr1^lox/loxRb1/Trp53/Rbl2 murine cells infected with Ad-Cre in the flanks of B6/129S F1 hybrid mice (Jackson Laboratory). To induce FGFR1 expression in Fgfr1-preSC following implantation, mice were fed doxycycline diet (625mg/kg, Envigo). Mice were maintained according to guidelines from the National Institutes of Health. Animal procedures were approved by the Institutional Animal Care and Use Committee at the University of Virginia.

Kim K.B., Kim Y., Rivard C.J., Kim D.W, & Park K.S. (2020). FGFR1 is critical for RBL2 loss-driven tumor development and requires PLCG1 activation for continued growth of small cell lung cancer. Cancer research, 80(22), 5051-5062.

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Doxycycline-Activated Neuronal Populations

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Mice were placed on Doxycycline diet (625mg/kg, Envigo, Indianapolis, IN) ad libitum 1 day prior to viral infusion. The diet was discontinued 2 days before the Context A retrieval test to label activated FRAM or NRAM populations. The following day, the Dox diet was resumed until the end of the experiment.

Ren L.Y., Cicvaric A., Zhang H., Meyer M.A., Guedea A.L., Gao P., Petrovic Z., Sun X., Lin Y, & Radulovic J. (2022). Stress-Induced Changes of the Cholinergic Circuitry Promote Retrieval-Based Generalization of Aversive Memories. Molecular psychiatry, 27(9), 3795-3805.

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Subcutaneous Tumor Xenograft Assay in NSG Mice

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Eight weeks old NSG mice (18–21 g) were randomized into different cages and subcutaneously injected with 1 × 10⁶ of YTN5, YTN16, YTN3, or YTN3^shYWHAG cells resuspended in Matrigel, respectively. All YTN3^shYWHAG mice were fed a doxycycline diet (625 mg kg⁻¹) (Envigo, USA) 2 weeks postinjection. The tumors were monitored and measured with a Vernier caliper every other day. The sample size (n = 10 mice/treatment group) was determined using a power analysis. At endpoint, the mice were euthanized with carbon dioxide and primary and secondary tumors were harvested. The tumors were snap frozen in liquid nitrogen and stored at −80 °C until further analysis or fixed in 4% paraformaldehyde for tissue processing. All procedures in the animal experimentation were performed according to the Nanyang Technological University's Institutional Animal Care and Use Committee guidelines (A19032, A19034).

Lee J.X., Tan W.R., Low Z.S., Lee J.Q., Chua D., Yeo W.D., See B., Vos M.I., Yasuda T., Nomura S., Cheng H.S, & Tan N.S. (2023). YWHAG Deficiency Disrupts the EMT‐Associated Network to Induce Oxidative Cell Death and Prevent Metastasis. Advanced Science, 10(31), 2301714.

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Evaluating LRP8 Knockdown Effects on Tumor Growth

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The animal studies were conducted following the protocols approved by the University Committee on the Use and Care of Animals at the University of Michigan. Female NOD/SCID mice (Jackson Laboratory, Bar Harbor, ME, USA) were used to evaluate the effects of LRP8 knockdown on tumor growth and tumorigenicity. Briefly, 5000 SUM149 cells carrying doxycycline-inducible control or LRP8 shRNA were injected into the inguinal mammary fat pad of 6–8-week-old mice. Doxycycline diet (625 mg/kg) (Envigo, Haslett, MI, USA) was given to mice starting from 5 weeks after implantation when palpable tumors were observed. At the end of tumor growth monitoring, tumors were harvested and dissociated by using Tumor Dissociation Kit, human (Miltenyi Biotec, Auburn, CA, USA), and then DAPI and H-2Kd (BD Biosciences) double-negative live human cancer cells were sorted by flow cytometry. Limiting dilution assay was conducted by inoculating the sorted and serially diluted cancer cells (2,500, 500 and 100 cells/inoculation) into the inguinal mammary fat pad of tumor-free mice. Tumor formation was monitored for 3 months. The frequency of tumor-initiating cells was calculated by using Extreme Limiting Dilution Analysis (ELDA) [25 ].

Lin C.C., Lo M.C., Moody R., Jiang H., Harouaka R., Stevers N., Tinsley S., Gasparyan M., Wicha M, & Sun D. (2018). Targeting LRP8 inhibits breast cancer stem cells in triple-negative breast cancer. Cancer letters, 438, 165-173.

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Syngeneic Mouse Mammary Tumor Transplantation

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Mammary glands from the donor strain (female virgin mice 7–9 weeks old) were harvested, singularized and seeded on a 2D collagen coated six-well plate (BD, Cat. # 356400). Cells were fed with mammary gland specific media (Promocell, Cat.#21210), plus Doxycycline hyclate (Sigma, D9891) overnight. The next day, tumor cells were extracted, counted, and resuspended in a solution of PBS and Matrigel (Corning, 356231). A mixture of 150,000 cells in 40 µl of media were injected into the donor strain using the 50 µl syringe (Hamilton, 705 N ga22s/51 mm/pst2). Female virgin RAG1 animals (from 4 to 8 weeks), were fed with Doxycycline diet (Envigo, TD.01306) at least 2 days prior to transplantation and for as long as tumor growth was desired. Animals were anesthetized using Xylazine/Ketamine before the surgery procedure. Closure of the wound was done by manual suture and no further post operational issues or inflammatory responses were observed.

Ozturk M.S., Montero M.G., Wang L., Chaible L.M., Jechlinger M, & Prevedel R. (2021). Intravital mesoscopic fluorescence molecular tomography allows non-invasive in vivo monitoring and quantification of breast cancer growth dynamics. Communications Biology, 4, 556.

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Athymic and NSG Mouse Housing Protocol

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All animal studies were performed in accordance with UTSW Institutional Animal Care and Use Committee guidelines (Animal protocol number #2017-102099). 4-5 weeks old female Athymic mice (Hsd: Athymic nude mice-Foxn1^nu) and NSG mice (NOD.Cg-Prkdc^scidIl2rg^tm1Wjl/SzJ) were purchased from Envigo (#069) and Jackson laboratory (#005557) were allowed to acclimatize to housing condition in animal facility for 1 week before use for the experiments. Animals used for all the experiment were aged between 5-6 weeks. All mice were housed in the UT Southwestern animal facility room maintained in a 12/12-hour light/dark cycle at a temperature of 20-26°C with 30-50% humidity and fed with standard Teklad diet (Envigo, #2916). In doxycycline inducible shRNA mediated knockdown, mice were fed with doxycycline diet (Envigo, #TD.08541). Ad libitum access to food and water was provided to mice at all times. Animal health was monitored once daily throughout the timeline of experiment.

Parida P.K., Marquez-Palencia M., Nair V., Kaushik A.K., Kim K., Sudderth J., Quesada-Diaz E., Cajigas A., Vemireddy V., Gonzalez-Ericsson P.I., Sanders M.E., Mobley B.C., Huffman K., Sahoo S., Alluri P., Lewis C., Peng Y., Bachoo R.M., Arteaga C.L., Hanker A.B., DeBerardinis R.J, & Malladi S. (2022). Metabolic diversity within breast cancer brain-tropic cells determines metastatic fitness. Cell metabolism, 34(1), 90-105.e7.

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