The human urinary bladder transitional cell carcinoma cell lines T24 (RRID: CVCL_0554), UM-UC-3 (RRID: CVCL_1783) were purchased from ATCC, RT112 (RRID: CVCL_1670) was purchased from German Collection of Microorganisms and Cell Cultures GmbH (DSMZ) and UM-UC-1 (RRID: CVCL_2743) was purchased from European Collection of Authenticated Cell Cultures (ECACC). Human normal bladder epithelial cell line SV-HUC-1 (RRID: CVCL_3798) was purchased from ATCC. Murine transitional cell carcinoma cell line MB49 (RRID: CVCL_7076) was purchased from Millipore, and murine bladder epithelial cells (MBEC) were purchased from Procell (catalog no. CP-M058). Human lymphatic endothelial cells (HLEC) were obtained from ScienCell Research Laboratories. All cells were maintained in a humidified incubator with 5% CO2 at 37°C. The T24, RT112, and UM-UC-1 cells were cultured in RPMI 1640 medium (Gibco, catalog no. C11875500BT). The UM-UC-3 and MB49 cells were cultured in DMEM (Gibco, catalog no. C11995500BT). The SV-HUC-1 cells were cultured in Ham's F12K medium (Gibco, catalog no. 21127022). All medium was supplemented with 10% FBS (BI, catalog no. 04–001–1ACS). The HLECs were cultured in endothelial cell medium (ECM) with 5% FBS (ScienCell Research Laboratories, catalog no. 1001). The authentication and Mycoplasma testing of all cell lines were qualified.
Um uc 1
Manufactured by Thermo Fisher Scientific
Sourced in United States
The UM-UC-1 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is a device designed for performing various laboratory tasks and experiments. The core function of the UM-UC-1 is to provide a controlled environment for conducting research and analysis. No further details on the intended use or specific applications of this product can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Rt112, by Thermo Fisher Scientific (2 mentions)
Um uc 3, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Umuc3, by American Type Culture Collection (1 mentions)
Sv huc 1, by American Type Culture Collection (1 mentions)
Sv huc 1, by Thermo Fisher Scientific (1 mentions)
Ham s f 12k medium, by Thermo Fisher Scientific (1 mentions)
Rt112, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hlecs, by ScienCell (1 mentions)
Sv huc 1, by Macgene (1 mentions)
Ham s f 12 k medium, by Macgene (1 mentions)
Sv huc 1, by Procell (1 mentions)
Umuc3, by Procell (1 mentions)
Rt112, by Procell (1 mentions)
2 protocols using um uc 1
1
Bladder Cancer Cell Lines Cultivation
2
Bladder Cancer Cell Line Transfection
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Human ureteral epithelial cells SV-HUC-1, BC cell lines including UM-UC-1, UM-UC-3, RT4, RT112 and T24 were purchased from Procell Life Sciences (Wuhan, China). SV-HUC-1 cells were cultured in Ham’s F-12 K medium (Macgene, China) supplemented with 10% fetal bovine serum (FBS) (Gibco, MA, USA). UM-UC-1, UM-UC-3, RT4, RT112 and T24 cells were cultured in RPMI-1640 medium (Gibco, MA, USA) supplemented with 10% FBS. All cells were cultured in 5% CO2 at 37 °C.
The si-PFK-1 and PFK-1 overexppressing plasmids and their negative control were synthesized by Gemma Shanghai. UM-UC-1 and RT112 cells were grown at 90% fusion. Transfection was performed using Lipofectamine 3000 regent according to the instructions. The culture medium was changed 6 h after transfection, and PCR was performed to detect transfection efficiency at 24–48 h after transfection.
The si-PFK-1 and PFK-1 overexppressing plasmids and their negative control were synthesized by Gemma Shanghai. UM-UC-1 and RT112 cells were grown at 90% fusion. Transfection was performed using Lipofectamine 3000 regent according to the instructions. The culture medium was changed 6 h after transfection, and PCR was performed to detect transfection efficiency at 24–48 h after transfection.
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