ABCB1-overexpressing KB-C2 cells were incubated with TAK-243 for up to 10 days. The total soluble protein lysates were prepared, subjected to SDS-PAGE, and transferred to PVDF membranes [44 (link)]. To quantify the expression level of ABCB1, membranes were probed with anti-ABCB1 monoclonal antibody (C219) (MA1-26528, Thermo Fisher Scientific Inc., Waltham, MA, USA). The loading control GAPDH was probed by anti-GAPDH monoclonal antibody (GA1R) (MA5-15738-D680, Thermo Fisher Scientific Inc., Waltham, MA, USA). Mouse IgG HRP conjugates (#7076, Cell Signaling Technology Inc., Danvers, MA, USA) were used as secondary antibody. To visualize the bands, ECL detection reagents were used (Thermo Fisher Scientific Inc., Waltham, MA, USA). The protein band densitometry was measured using ImageJ software (NIH, MD, USA).
Anti gapdh monoclonal antibody ga1r
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-GAPDH monoclonal antibody (GA1R) is a laboratory reagent used for the detection and quantification of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a commonly used internal control or reference protein in various biological assays and analyses.
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Lab products found in correlation
2 protocols using anti gapdh monoclonal antibody ga1r
1
Quantifying ABCB1 Expression in TAK-243-Treated KB-C2 Cells
2
Comprehensive Western Blot Analysis
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Western blotting was performed as previously described (Seo et al., 2017 (link)). Affinity-purified rabbit polyclonal anti-CPNE7 and anti-DSP antibodies were produced as previously described (Lee et al., 2010 (link); Lee et al., 2011 (link)). Anti-LC3 B polyclonal antibody (Abcam, ab48394), anti-TAU monoclonal antibody (TAU-5; Thermo Fisher Scientific, AHB0042), anti-phospho-mTOR (mechanistic target of rapamycin kinase) polyclonal antibody (Cell Signaling Technology, 5536), anti-DMP-1 (dentin matrix protein 1) polyclonal antibody (Abcam, ab103203), anti-NESTIN monoclonal antibody (10C2; Thermo Fisher Scientific, MA1-110), and anti-GAPDH monoclonal antibody (GA1R; Thermo Fisher Scientific, MA5-15738) were purchased from the respective companies. Protein loading was assessed by the expression of GAPDH. All reactions were performed in triplicate. Semi-quantitative analysis were performed using ImageJ software (National Institute of Health).
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