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2 protocols using 3 n desmethyl caffeine paraxanthine

1

In Vitro Cytochrome P450 Assay

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Lurasidone and iloperidone were obtained from TargetMol (Boston, USA). Caffeine, 3-N-desmethyl Caffeine (paraxanthine), diclofenac, 4′-hydroxydiclofenac, bufuralol, 1′-hydroxybufuralol, NADP, NADPH, glucose-6-phosphate, glucose-6-phosphate-dehydrogenase, MgCl2, KCl, ZnSO4, Trizma base and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma (St. Louis, USA). Testosterone and 6β-Testosterone were from Steraloids (Newport, USA). All the organic solvents with HPLC purity were supplied by Merck (Darmstadt, Germany). Pooled human liver microsomes and microsomes from baculovirus-infected insect cells expressing human CYP1A2, CYP2C9, CYP2C19, CYP2D6 and CYP3A4 (supersomes) were provided by Corning (Woburn, USA).
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2

In Vitro CYP Metabolism Assay

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Asenapine, caffeine, 3-N-desmethyl caffeine (paraxanthine), diclofenac, 4′-hydroxydiclofenac, bufuralol, 1′-hydroxybufuralol, NADP, NADPH, glucose-6-phosphate, glucose-6-phosphate-dehydrogenase, MgCl2, KCl, ZnSO4, Trizma base, and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma (St. Louis, USA). Testosterone and 6β-Testosterone were from Steraloids (Newport, USA). All the organic solvents with HPLC purity were supplied by Merck (Darmstadt, Germany). Pooled human liver microsomes and microsomes from baculovirus-infected insect cells expressing human CYP1A2, CYP2C9, CYP2C19, CYP2D6, and CYP3A4 (Supersomes) were provided by Corning (Woburn, USA).
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