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Fibroblast growth factor 21

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Fibroblast growth factor 21 is a protein that plays a role in regulating metabolism. It is commonly used in laboratory settings for research purposes.

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Lab products found in correlation

Cholesterol, by Thermo Fisher Scientific (3 mentions) Insulin, by Merck Group (3 mentions) β hydroxybutyrate, by Abcam (3 mentions) Free fatty acids, by Roche (3 mentions) Leptin, by R&D Systems (3 mentions) Adiponectin, by ALPCO (2 mentions) Contour glucometer, by Bayer (2 mentions) Free t3, by Eagle Biosciences (2 mentions) Free t4, by Eagle Biosciences (2 mentions) Adiponectin, by Merck Group (1 mentions) Triglycerides, by Horiba (1 mentions) Microtainer serum separator tube, by BD (1 mentions) D lactate, by Abcam (1 mentions)

3 protocols using fibroblast growth factor 21

1

Metabolic Profiling in Mice

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Intraperitoneal glucose (2 g/kg for chow-fed mice, 1 g/kg for HFD-fed mice, with AUC calculated from 0 mg/dl) tolerance tests were performed at 1200, following a 4 hour fast. Glucose was measured with a Glucometer Contour (Bayer, Mishawaka, IN). Liver triglyceride was measured as glycerol after KOH hydrolysis (Cayman Chemical, #10010755). Serum was collected at 0900 by tail bleed 2 days before euthanasia for fed glucose, Insulin, FFA, cholesterol, and triglycerides measurement. Serum for other measurements was taken from anesthetized (100 mg/kg ketamine and 10 mg/kg xylazine, ip) mice by retro-orbital bleed at euthanasia and frozen until assayed using the following colorimetric assays: Free fatty acids (Roche Diagnostics Gmbh, Mannheim, Germany), triglycerides (Pointe Scientific Inc., Canton, MI), cholesterol (Thermo Scientific, Middletown, VA), and β-hydroxybutyrate (BioVision, Milpitas, CA). Insulin (Millipore, St. Charles, MO) was measured by RIA. Leptin (R&D Systems, Minneapolis, MN), adiponectin (ALPCO, Salem, NH), fibroblast growth factor 21 (Millipore), free T3 and free T4 (Eagle Biosciences, Nashua, NH) were measured by ELISA.
Xiao C., Goldgof M., Gavrilova O, & Reitman M.L. (2015). Anti-obesity and metabolic efficacy of the β3-adrenergic agonist, CL316243, in mice at thermoneutrality compared to 22°C. Obesity (Silver Spring, Md.), 23(7), 1450-1459.
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2

Serum Metabolite and Hormone Profiling

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Blood from retro-orbital bleeding was put in BD Microtainer Serum Separator Tubes (Becton, Dickinson and Company, Franklin Lakes, NJ), allowed to clot for 10 minutes at room temperature, spun at 10,000 rpm for 6 minutes, and serum was frozen until assayed. Free fatty acids (Roche Diagnostics Gmbh, Mannheim, Germany), triglycerides (Pointe Scientific Inc., Canton, MI), cholesterol (Thermo Scientific, Middletown, VA), β-hydroxybutyrate (BioVision, Milpitas, CA), and D-lactate (BioVision, Milpitas, CA) were measured by colorimetric assays. Serum glucose was measured by Glucometer Contour. Insulin (Millipore, St. Charles, MO), adiponectin (Millipore, St. Charles, MO), Insulin-like growth factor 1 (ALPCO, Salem, NH), T3 (DiaSorin Inc, Stillwater, MN), and T4 (DiaSorin Inc, Stillwater, MN) were measured by RIA. Leptin (R&D Systems, Minneapolis, MN) and fibroblast growth factor 21 (Millipore, St. Charles, MO) were measured by ELISA. All assays were conducted as per manufacturer's protocol.
Ravussin Y., Xiao C., Gavrilova O, & Reitman M.L. (2014). Effect of Intermittent Cold Exposure on Brown Fat Activation, Obesity, and Energy Homeostasis in Mice. PLoS ONE, 9(1), e85876.
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3

Metabolic Profiling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Intraperitoneal glucose (2 g/kg for chow-fed mice, 1 g/kg for HFD-fed mice, with AUC calculated from 0 mg/dl) tolerance tests were performed at 1200, following a 4 hour fast. Glucose was measured with a Glucometer Contour (Bayer, Mishawaka, IN). Liver triglyceride was measured as glycerol after KOH hydrolysis (Cayman Chemical, #10010755). Serum was collected at 0900 by tail bleed 2 days before euthanasia for fed glucose, Insulin, FFA, cholesterol, and triglycerides measurement. Serum for other measurements was taken from anesthetized (100 mg/kg ketamine and 10 mg/kg xylazine, ip) mice by retro-orbital bleed at euthanasia and frozen until assayed using the following colorimetric assays: Free fatty acids (Roche Diagnostics Gmbh, Mannheim, Germany), triglycerides (Pointe Scientific Inc., Canton, MI), cholesterol (Thermo Scientific, Middletown, VA), and β-hydroxybutyrate (BioVision, Milpitas, CA). Insulin (Millipore, St. Charles, MO) was measured by RIA. Leptin (R&D Systems, Minneapolis, MN), adiponectin (ALPCO, Salem, NH), fibroblast growth factor 21 (Millipore), free T3 and free T4 (Eagle Biosciences, Nashua, NH) were measured by ELISA.
Xiao C., Goldgof M., Gavrilova O, & Reitman M.L. (2015). Anti-obesity and metabolic efficacy of the β3-adrenergic agonist, CL316243, in mice at thermoneutrality compared to 22°C. Obesity (Silver Spring, Md.), 23(7), 1450-1459.
+ Open protocol
+ Expand

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