Avance 400ft

IR spectra were recorded with a NICOLET iS50 FT-IR spectrometer (Thermo Scientific, Waltham, MA, USA). NMR data were collected on Bruker Avance-400FT and Avance-600 NMR spectrometers (Bruker Corporation, Billerica, MA, USA). GC–MS analyses were performed on an Agilent 7890A GC system connected to an Agilent 5975 mass spectrometer with a HP-5MS column (30 m × 250 µm × 0.25 µm, Agilent Technologies, Santa Clara, CA, USA). HREIMS spectrum was collected on a Hybrid Quadrupole-Orbitrap GC-MS/MS System (Thermo Scientific, Waltham, MA, USA) equipped with a TraceGOLD TG-5HT column (30 m × 250 µm × 0.1 µm, Thermo Scientific, Waltham, MA, USA). Size-exclusion chromatography was carried out with Sephadex LH-20 (GE Healthcare, Chicago, IL, USA) columns. HPLC analysis was performed on an Agilent 1260 series (Agilent Technologies, Santa Clara, CA, USA) with a C18 RP-column (Extend-C18, 250 × 4.6 mm, 5 μm, Agilent Technologies, Santa Clara, CA, USA). Semi-preparative HPLC was performed on an SSI 23201 system (Scientific Systems Inc., State College, PA, USA) with a YMC-Pack ODS-A column (250 × 10 mm, 5 µm, YMC CO., LTD. Shimogyo-ku, Kyoto, Japan). All fermentations were carried out in MQD-B1R shakers (Minquan Instrument Co., Ltd., Shanghai, China).

All chemicals and reagents were purchased from Sigma-Aldrich (China-mainland) and Energy Chemical (China-mainland) and used directly without further purification. All solvents were distilled before use, including DCM (CaH₂) and acetone (4 Å MS). Dry DMF, CH₃CN, and CH₃OH were purchased from Innochem (Beijing, China). ¹H NMR and ¹³C NMR spectra were recorded on a Bruker Avance-400 FT nuclear magnetic resonance spectrometer. Chemical shifts were reported relative to the reference chemical shift of the NMR solvent. The synthesis route of MGP from compound 1 to compound 6 (MGP) is described in Figure 1—source data 1.