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2 protocols using anti p53 7f5

1

Comprehensive Western Blotting Antibody Panel

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The following antibodies used in Western blotting were purchased from Cell Signaling Technology (Danvers, MA, USA): anti-HA-tag (6E2) (1:1000, #2367, Cell Signaling Technology), anti-p53 (7F5) (1:1000, #2527, Cell Signaling Technology), anti-DDB-1 (D4C8) (1:1000, #6998, Cell Signaling Technology), anti-DDB-2 (D4C4) (1:1000, #5416, Cell Signaling Technology) anti-ERCC1 (1:1000, #3885, Cell Signaling Technology), and anti-XPC (D1M5Y) (1:1000, #14768, Cell Signaling Technology). The Polyclonal anti-KMT4/DOT1L (1:2000, ab72454, Abcam) antibody, anti-XPA (1:2000, ab85914, Abcam), polyclonal di-methylated H3K79 antibody (1:1000, ab3594, Abcam), polyclonal to mono-methylated H3K79 antibody (1:500, ab2886, Abcam), and polyclonal tri-methylated H3K79 antibody (1:1000, ab2621, Abcam) were purchased from Abcam. TFIIH p62 Antibody (1:500, Q-19) was purchased from Santa Cruz Biotechnologies, Inc. Other antibodies include monoclonal Anti-β-Actin-Peroxidase antibody (1:25000, AC-15, Sigma Aldrich), monoclonal ANTI-FLAG® M2 antibody (1:1000, Sigma Aldrich), and anti-H3 (1:500, 865R2, Thermo Fisher Scientific Inc.). Most important uncropped blot images are shown in Supplementary Fig. 11.
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2

Mitochondrial Dynamics and Signaling Pathways

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Amlodipine was from Tokyo Chemical Industry Co. Ltd. Mdivi-1, sildenafil, atenolol, propranolol, metoprolol, thiocarbohydrazide solution, durcupan resin, c-Myc peptide, and GTP-agarose were from Sigma-Aldrich. Dulbecco's modified Eagle's medium (DMEM), diltiazem, nifedipine, nisoldipine, nicardipine, nitrendipine, verapamil, losartan, uranium acetate, anti-Myc, and anti--actin were from Wako. MitoTracker Green FM, MitoTracker Red CM-H 2 XRos, and MitoSox Red were from Invitrogen. Collagenase II was from Worthington. Osmium tetroxide was from Electron Microscopy Science. Bovine serum albumin (BSA), sodium cacodylate, and phenol red-free DMEM were from Nacalai Tesque. Anti-Drp1 (H-300), anti-GAPDH (FL-335), anti-SOD2 (Mn-SOD: N-20), anti-Mfn1 (H-65), and anti-filamin 1 (FLNa: H-300 and E-3) were from Santa Cruz Biotechnology. Anti-p53 (7F5) and horseradish peroxidase (HRP)-conjugated anti-mouse and rabbit IgG were from Cell Signaling Technology. Anti-tafazzin, anti-HIF-1, anti-sarcomeric -actinin (EA-53), and anti-Mfn2 were from Abcam. Anti-Opa1 was from BD Biosciences. Anti-HO-1 was from Enzo Life Sciences. EGTA and Hepes were from Dojindo.
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