Advance 3 600 mhz nmr spectrometer

¹H NMR spectra were recorded at the University of Copenhagen (Department of Food Science) using a Bruker Advance III 600 MHz NMR spectrometer equipped with a 5 mm broadband inverse RT (BBI) probe, automated tuning and matching accessory (ATMA) and cooling unit BCU-05, and an automated sample changer (Sample Jet, Bruker Biospin, Rheinstetten, Germany) with sample cooling (278 K) and preheating stations (298 K). Data acquisition and processing were carried out using TOPSPIN 3.5 PL6 (Bruker Biospin, Rheinstetten, Germany). The Icon NMR (Bruker Biospin, Rheinstetten, Germany) interface was used to control the automation of the overall measurement procedure. Automatic tuning and matching, lock, and shimming were performed using TOPSPIN 3.5 PL6. The standard pulse sequence with water suppression (noesygppr1d) was employed for measuring the one-dimensional ¹H NMR spectra. A total of 32 scans were acquired after 4 dummy scans, and the generated free induction decays (FIDs) were collected into 128 k data points using a spectral width of 20 ppm. Acquisition time, recycle delay, and mixing time were set to 2.72, 4.0, and 0.01 s, respectively. The receiver gain was set to 90.5. A more detailed description of NMR analysis has been reported in a previous paper [16 (link)].