Reagents for polyacrylamide gel electrophoresis

Fetal calf serum, penicillin/streptomycin, and basal Eagle's medium were purchased from GIBCO, Invitrogen (Carlsbad, CA, USA). Dihydroethidium (DHE), MitoTracker green, and MitoTracker red CMH₂XRos were purchased from Molecular Probes, Invitrogen (Carlsbad, CA, USA). Poly-l-lysine, trypsin, trypsin inhibitor, DNAse, cytosine arabinoside, DMSO (dimethyl sulfoxide), staurosporine, MitoTEMPO, and reagents for polyacrylamide gel electrophoresis (PAGE) were acquired from Sigma (St. Louis, MO, USA). Protease inhibitor cocktail tablets (Complete) were purchased from Roche (Mannheim, Germany), and phosphatase inhibitor minitablets were obtained from Thermo Scientific (Rockford, USA). ProSieve Quad Color Protein Marker was purchased from Lonza (Rockland, Maine, USA). Polyvinylidene fluoride (PVDF) membranes and Immobilon Western HRP substrate were acquired from Millipore (Concord Road, Billerica, MA, USA). Antibodies against Drp1, Drp1 (Ser616), and GAPDH were from Cell Signaling Technology (Danvers, MA, USA); peroxidase-conjugated anti-mouse was purchased from Jackson ImmunoResearch (West Grove, PA, USA).

9-Fluorenylmethoxycarbonyl (Fmoc) and tert-butoxycarbonyl (Boc) protected amino acids, [4-[α-(20,40-dimethoxyphenyl) Fmoc-aminomethyl] phenoxy] acetamidonorleucyl-MBHA resin (Rink amide MBHA resin), and 2-(1Hbenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) were purchased from NovaBiochem and ABCR. Other chemicals for peptide synthesis were purchased from Fisher, Merck, AlfaAesar, or Aldrich. All chemicals were used as provided. CpG and control ODNs were purchased from Invivogen. Paired antibodies and recombinant proteins of IFNγ and IL-12 were obtained from R&D systems, that of IL-6 from eBioscience and TNFα from Life Technologies. All cell culture and ELISA reagents were purchased from Life Technologies, except non-essential amino acid solution (Sigma Aldrich). Reagents for polyacrylamide gel electrophoresis were obtained from Sigma Aldrich. Fluorescently labelled antibodies were obtained from BD Pharmingen (B220 and CD11c) and eBioscience (F4/80, CD40, CD86).