Cd39 clone a1

Manufactured by BioLegend

CD39 (clone A1) is a monoclonal antibody that binds to the CD39 protein. CD39 is an ectonucleotidase enzyme that catalyzes the hydrolysis of extracellular ATP and ADP to AMP. This antibody can be used to detect and study CD39-expressing cells.

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Lab products found in correlation

3 protocols using cd39 clone a1

Murine and Human Monoclonal Antibody Panel

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Murine specific monoclonal antibodies: CD3 (clone 17A2), CD11a (clone M17/4), CD18 (clone M18/2), CD16/CD32 (Fc Block, clone 2.4G2), CD19 (clone 6D5), CD39 (clone Duha59), CD44 (clone IM7), CD47 (clone miap301), CD49d (clone R1-2), CD54 (clone YN1/1.7.4), CD81 (clone Eat-2), CD98 (clone RL388), CD130 (clone 4H1B35), CD138 (clone 281-2), CD155 (clone TX56), CD172a (clone P84), CD205 (clone NLDC- 145), CD229 (clone Ly9ab3), CD267 (clone 8F10), CD274 (clone 10F.9G2), CD319 (clone 4G2), CD326 (clone G8.8), IgA (clone mA-6E1), IgM (clone RMM-1), LAG-3 (Clone eBioC9B7W), SCA-1 (clone D7) and human specific monoclonal antibodies: CD3 (clone UCHT1), CD14 (clone M5E2), CD19 (clone SJ25C1), CD20 (clone 2H7), CD27 (clone L128), CD39 (clone A1), CD81 (clone 5A6), CD130 (clone 2E1B02), CD138 (clone MI15), and CD326 (clone 9C4) were purchased from BioLegend, Miltenyi Biotec, BD Biosciences, Thermo Fisher or produced in house. LEGENDScreen™ Mouse PE Kit was purchased from BioLegend.

Dang V.D., Mohr E., Szelinski F., Le T.A., Ritter J., Hinnenthal T., Stefanski A.L., Schrezenmeier E., Ocvirk S., Hipfl C., Hardt S., Cheng Q., Hiepe F., Löhning M., Dörner T, & Lino A.C. (2022). CD39 and CD326 Are Bona Fide Markers of Murine and Human Plasma Cells and Identify a Bone Marrow Specific Plasma Cell Subpopulation in Lupus. Frontiers in Immunology, 13, 873217.

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Comprehensive Intracellular Immune Profiling

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Intracellular staining was done using the eBioscience Foxp3 staining kit with antibodies for FoxP3 (206D, BioLegend), GzmB (GB11, eBioscience). In some experiments, loss of cell viability was assessed via a viability dye (Invitrogen) before fixation. In other experiments, stimulated cells were labeled with annexin V (BD Biosciences). In some experiments, the cells were also surface stained for CTLA4 (clone BN13, BD Biosciences), PD-L1 (clone 29F.2A3 BioLegend), CD39 (clone A1 BioLegend), or LAG3 (clone 17B4, Enzo Life Sciences). Data were acquired on a FACS Calibur or LSRII flow cytometer (BD Biosciences) using CellQuest software and analyzed using FlowJo software (Tree Star).

Bhela S., Kempsell C., Manohar M., Dominguez-Villar M., Griffin R., Bhatt P., Kivisakk-Webb P., Fuhlbrigge R., Kupper T., Weiner H, & Baecher-Allan C. (2015). Non-apoptotic and extracellular activity of Granzyme B mediates resistance to Treg suppression by HLA-DRnegCD25hiCD127lo Tregs in multiple sclerosis and in response to IL-6. Journal of immunology (Baltimore, Md. : 1950), 194(5), 2180-2189.

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Multiparametric Flow Cytometry of CAR-T Cells

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Human HER2 and PD-L1 expression on tumor cells was detected using human HER2-PE-CY7 (clone 24D2, Biolegend) and PD-L1-APC (clone MIH1, eBioscience). HER2 4D5 CAR was detected using the anti-trastuzumab idiotype Alexa Fluor 647-conjugated antibody (clone 2661E, R&D system). HER2 4D5 CAR was also detected using human recombinant HER2 protein conjugated with Alex Fluor 647. Human T cells surface phenotype and transduction efficiency were assessed using the following antibodies: NGFR-FITC (clone ME20.4, Biolegend), Q8 (clone QBEND/10, ThermoFisher), CD45-AF700 or CD45-BV605 (clone HI30, Biolegend), CD3-APC (clone SK7, Biolegend), CD4-PerCP (clone SK3, Biolegend), CD4-BB700 (clone SK3, BD Bioscience), CD8-BV510 (clone SK1, Biolegend), CD27-PE-CY7 (clone M-T271, Biolegend), CD28-BV605 (clone CD28.2, Biolegend. Expression of T cell inhibitory receptors was analyzed using PD-1-BV421 or PD-1-BV605 (clone EH12.2H7, Biolegend), TIM-3-BV605 or TIM-3-PE-CY-7 (clone F38-2E2, Biolegend), CD39 (clone A1, Biolegend), LAG-3-BV711 (clone 11C3C65, Biolegend). Live/dead discrimination was determined using LIVE/DEAD fixable Near-IR dead cell stain kit (ThermoFisher). Flow cytometry results were analyzed using Kaluza software (Beckman Coulter).

Yang Z., Li L., Turkoz A., Chen P., Harari-Steinfeld R., Bobbin M., Stefanson O., Choi H., Pietrobon V., Alphson B., Goswami A., Balan V., Kearney A., Patel D., Yang J., Inel D., Vinod V., Cesano A., Wang B., Roh K.H., Qi L.S, & Marincola F.M. (2021). Contextual reprogramming of CAR-T cells for treatment of HER2+ cancers. Journal of Translational Medicine, 19, 459.

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