Anti enos mouse polyclonal antibody

Manufactured by BD

The Anti-eNOS mouse polyclonal antibody is a laboratory reagent used for the detection and analysis of endothelial nitric oxide synthase (eNOS) in various biological samples. This antibody is produced in mice and is designed to specifically bind to eNOS, a key enzyme involved in the production of nitric oxide, which plays a critical role in vascular function and regulation.

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Lab products found in correlation

β actin, by Cell Signaling Technology (2 mentions) Anti gapdh rabbit polyclonal antibody, by Santa Cruz Biotechnology (1 mentions) Anti arginase 1, by Cell Signaling Technology (1 mentions) Anti stat6, by Cell Signaling Technology (1 mentions) Anti pkg, by Cell Signaling Technology (1 mentions) Anti vasp, by Cell Signaling Technology (1 mentions) Amplex red glucose glucose oxidase assay kit, by Thermo Fisher Scientific (1 mentions) Anti phospho nf κb p65 ser536, by Cell Signaling Technology (1 mentions) Anti mac2, by Cedarlane (1 mentions)

Close spelling variants of this product

Mouse anti-eNOS Anti-eNOS antibody ENOS antibody Anti-eNOS Mouse anti

2 protocols using anti enos mouse polyclonal antibody

Signaling Pathway Antibodies and Assays

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Anti–phosphorylation (p) inhibitor of B (IκB)-α, anti-PKG, anti-VASP, and anti–p-VASP (Ser 239), anti-Arginase1, anti-STAT6, anti–p-STAT6 (Tyr 641), and β-actin antibodies were from Cell Signaling Technology (Beverly, MA). Anti-eNOS mouse polyclonal antibody was obtained from BD Biosciences (Lexington, KY). Anti-GAPDH rabbit polyclonal antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Biosource total Akt and p-Akt (Ser 473) ELISA kits were purchased from Invitrogen (Carlsbad, CA), and IRS2 and p-IRS2 ELISA kits were purchased from Cell Signaling Technology. Lactate was measured using an Amplex Red Glucose/Glucose Oxidase Assay Kit (Invitrogen). (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino] diazen-1-IM1,2-diolate (DETA-NO) was purchased from Enzo Life Sciences. DETA-NO was used within 24 h after the reconstitution.

Lee W.J., Tateya S., Cheng A.M., Rizzo-DeLeon N., Wang N.F., Handa P., Wilson C.L., Clowes A.W., Sweet I.R., Bomsztyk K., Schwartz M.W, & Kim F. (2015). M2 Macrophage Polarization Mediates Anti-inflammatory Effects of Endothelial Nitric Oxide Signaling. Diabetes, 64(8), 2836-2846.

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Protein Expression Analysis for Cell Signaling

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Anti-phospho-NF-κB p65 (ser536)(#3033), anti-NF-κB p65 (#8242), anti-iNOS (#2982), Anti-AMPKα (#2532), Anti-phosphoAMPKα(Thr172)(#2535), β-actin (#4967) antibodies were from Cell Signaling (Beverly, MA). Anti-eNOS mouse polyclonal antibody (#610296) was obtained from BD Bioscience (Lexington, KY). Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (FL-335) rabbit polyclonal antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-Mac2 (Cedarlane Laboratories). Total Akt, p-Akt (Ser 473), IRS-1, IRS-2 and p-IRS1, p-IRS-2 ELISA kits were purchased from Cell Signaling.

Dick B.P., McMahan R., Knowles T., Becker L., Gharib S.A., Vaisar T., Wietecha T., O’Brien K.D., Bornfeldt K.E., Chait A, & Kim F. (2020). Hematopoietic cell-expressed endothelial nitric oxide protects the liver from insulin resistance. Arteriosclerosis, thrombosis, and vascular biology, 40(3), 670-681.

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