Primary antibodies used in this study are as follows: mouse monoclonal anti-GFP (JL-8; Clontech, ab2313808), mouse monoclonal anti-FLAG M2 (Sigma-Aldrich, ab439702), mouse monoclonal anti-FLAG M2-peroxidase [horseradish peroxidase (HRP)] (Sigma-Aldrich, ab439702), rat monoclonal anti-HA (3F10; Roche, ab390917), mouse monoclonal anti-GST (GenScript, ab771432), mouse monoclonal anti-His (GenScript, ab914704), mouse monoclonal anti–c-Myc tag (Thermo Fisher Scientific, ab2556560), rabbit polyclonal anti-PEPC (Agrisera, ab2063166), rabbit polyclonal anti–histone H3 (Agrisera, AS10710), rabbit polyclonal anti-NPR1 (Agrisera, AS121854), rabbit polyclonal anti-PR1 (Agrisera, AS10687), rabbit polyclonal anti-EDS1 (Agrisera, AS132751), goat anti-mouse immunoglobulin G (IgG) (HRP) (Thermo Fisher Scientific, catalog no. G-21040), goat anti-rabbit IgG (HRP) (Thermo Fisher Scientific, catalog no. G-21234), and goat anti-rat IgG (HRP) (Thermo Fisher Scientific, catalog no. 31470).
Goat anti rat igg hrp
Manufactured by Thermo Fisher Scientific
Sourced in United States
Goat anti-rat IgG (HRP) is a secondary antibody that binds to rat immunoglobulin G (IgG) and is conjugated with horseradish peroxidase (HRP). It can be used in various immunoassay techniques, such as ELISA, Western blotting, and immunohistochemistry, to detect and quantify rat IgG in biological samples.
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Lab products found in correlation
Goat anti rabbit igg hrp, by Thermo Fisher Scientific (1 mentions)
As132751, by Agrisera (1 mentions)
As10687, by Agrisera (1 mentions)
As10 710, by Agrisera (1 mentions)
Pageblue, by Thermo Fisher Scientific (1 mentions)
Gradient polyacrylamide gel, by Bio-Rad (1 mentions)
Sds laemmli buffer, by Bio-Rad (1 mentions)
Anti flag l5, by BioLegend (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Chemidoc touch imaging system, by Bio-Rad (1 mentions)
Goat anti mouse igg hrp, by Thermo Fisher Scientific (1 mentions)
Mouse anti actr 1, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Mouse anti caga, by Santa Cruz Biotechnology (1 mentions)
Goat anti mouse igm cfl 488, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit igg, by Abcam (1 mentions)
4 protocols using goat anti rat igg hrp
1
Antibody Reagents for Plant Research
2
SDS-PAGE and Western Blot Analysis of Purified Antibody and Recombinant Granzyme B
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Purified antibody and recombinant human GrB (rGrB) (2906-SE, R&D Systems) were diluted in beta-mercaptoethanol-containing Laemmli SDS buffer (Bio-Rad), boiled, and resolved on a 4–15% gradient polyacrylamide gel (Bio-Rad) through SDS-PAGE. Coomassie blue staining was performed with PageBlue (Thermo Fisher) as indicated by the manufacturer. For western blotting, proteins were transferred to PVDF membrane (Millipore-Sigma), blocked with 5% non-fat dry milk for 1 h at RT, and incubated overnight at 4 °C with an appropriate antibody: anti-FLAG (L5, Biolegend), anti-human granzyme B (AF2906, R&D Systems), or peroxidase-anti-human IgG (709–035-149, Jackson ImmunoResearch). Blots were washed with 0.5% Tween 20 in PBS (PBST) and probed with either goat anti-rat IgG-HRP (A10549, Thermo Fisher) or bovine anti-goat IgG-HRP (805–035-180, Jackson ImmunoResearch) reagents for 1 h at RT. Following additional wash steps, blots were developed using SignalFire ECL (Cell Signaling Technology) and imaged on a ChemiDoc Touch imaging System (Bio-Rad).
3
Protein Expression Analysis via Western Blot
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For analyzing protein levels, either the samples from the co-immunoprecipitation or samples with ice-cold phosphate-buffered saline (PBS) and centrifugation-isolated hPdLFs were used. Further protein isolation and expression analysis by semi-dry Western blot was performed as previously described [81 (link)]. The following primary antibodies from Santa Cruz Biotechnology (Dallas, TX, USA) were used: rat anti-ALK1 (sc-101556, 1:100), mouse anti-ACTR-I (ALK2; sc-374523, 1:100), and rat anti-TGFβ-RI (ALK5; sc-101574, 1:100). The following secondary antibodies coupled to horseradish peroxidase (HRP) from Thermo Fisher Scientific (Carlsbad, CA, USA) were used: goat anti-mouse IgG HRP (#31430, 1:5000) and goat anti-rat IgG HRP (#31470, 1:5000).
4
Western Blot Analysis of Bacterial Virulence Factors
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The primary antibodies used for Western blot analysis were as follows: rat anti-integrin β1 clone AIIB2 (Merck, MABT409), rabbit anti-integrin α5 clone 5E18 ZooMAb (Sigma-Aldrich, ZRB1122-25UL), mouse anti-CD15s (sialyl Lewisx) (Santa Cruz, sc-32243), mouse anti-CagA (Santa Cruz, sc-28368), mouse anti-CagA phospho (Santa Cruz, sc-508), mouse anti-VacA (Santa Cruz, sc-32746), rabbit anti-p38 MAPK (Agrigo, ARG55258), rabbit anti-p38 MAPK phospho (Agrigo, ARG51850) rabbit anti-Cox-2 (Agrigo, ARG56421), goat anti-ATF-2 (Arigo, ARG63122), rabbit anti-ATF2 phospho (Agrigo, ARG42620), mouse anti-GAPDH (Agrigo, ARG10112). Furthermore, the secondary antibodies were as follows: donkey anti-goat IgG antibody (Arigo, ARG65352), goat anti-rabbit IgG (pre-adsorbed to 10 nm gold) (Abcam, ab27234), goat anti-rabbit IgG H&L (HRP) (Merck Millipore, AP132P), goat anti-mouse IgG H&L cross-adsorbed secondary antibody (Alexa Fluor 488) (Thermo Fisher, A-11001), goat anti-mouse IgG (HRP) (Biosciences, 554002) Goat anti-Rat IgG (HRP) (Thermo Fisher, 31470), goat anti-rabbit (Alexa Fluor 488) (Invitrogen A11034), goat anti-rat IgG H&L cross-adsorbed secondary antibody (Alexa Fluor 488) (Thermo Fisher, A-11006), and goat anti-mouse IgM CFL488 (Santa cruz, sc-395784).
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