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2 protocols using phospho c jun d47g9

1

Hepatocyte Stress Signaling Analysis

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Total liver lysates were analyzed by immunoblotting using antibodies against HBsAg (20-HR20, Fitzgerald), GADD153 (F-168, Santa Cruz), phospho-PERK (16F8, Cell Signaling), PERK (H-300, Santa Cruz), phospho-eIF2α (119A11, Cell Signaling), eIF2α (L57A5, Cell Signaling), β-actin (13E5, Cell Signaling), JNK2 (N-18, Santa Cruz), c-Jun (60A8, Cell Signaling), phospho-c-Jun (D47G9, Cell Signaling), phospho-SAPK/JNK (81E11, Cell Signaling), STAT3 (79D7, Cell Signaling), phospho-STAT3 (D3A7, Cell Signaling).
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2

Protein Analysis of Liver Samples

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Sample preparation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting was performed as described before [48 (link)]. Total liver lysates were analyzed by using antibodies against HBsAg (20-HR20, Fitzgerald, Acton, Ma, USA), phospho-PERK (16F8, Cell Signaling, Beverly, MA, USA), phospho-eIF2α (119A11, Cell Signaling), β-actin (13E5, Cell Signaling), c-Jun (60A8, Cell Signaling), phospho-c-Jun (D47G9, Cell Signaling), phospho-SAPK/JNK (81E11, Cell Signaling), STAT3 (79D7, Cell Signaling), phospho-STAT3 (D3A7, Cell Signaling).
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