Dried ripe fruits of Gardenia jasminoides J.Ellis (Fructus Gardeniae) (No. 180525) and the fermented ripe seeds of Glycine max (L.) Merr. (Semen sojae praeparatum) (No. 180716-1) were purchased from Tong Han Chun Tang Chinese Herbal Factory (Shanghai, Chinese). Authenticated by Professor Lu-Ping Qin, Fructus Gardeniae (Voucher number 2018082001) and Semen sojae praeparatum (Voucher number 2018082002) were deposited at the herbarium of pharmaceutical analysis, School of Pharmacy, Second Military Medical University, Shanghai, China. Fluoxetine was purchased from Eli Lilly and Company (China). Sodium carboxymethyl cellulose (CMC-Na) and sucrose were obtained from Sangon Biotech Co., Ltd. (Shanghai). LC-MS grade formic acid, acetonitrile, and methanol were supplied by Honeywell (United States). Urethane was purchased from meilunbio (China). Deionized water was collected by a laboratory water purification system (HITECH Instruments CO., Ltd.). Reactive Oxygen Species, Glutathione and Glutathione disulfide, catalase, glutathione reductase, glutathione peroxidase, and SOD assay kit were purchased from Beyotime (China).
Glutathione peroxidase
Manufactured by Beyotime
Sourced in China
Glutathione peroxidase is an enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides. It plays a crucial role in the cellular antioxidant defense system.
Automatically generated - may contain errors
Lab products found in correlation
Sod assay kit, by Beyotime (1 mentions)
Methanol, by Honeywell (1 mentions)
Acetonitrile, by Honeywell (1 mentions)
Lc ms grade formic acid, by Honeywell (1 mentions)
Catalase, by Beyotime (1 mentions)
Sodium carboxymethyl cellulose cmc na, by Sangon (1 mentions)
Fluoxetine, by Eli Lilly (1 mentions)
Sucrose, by Sangon (1 mentions)
Protein extraction kit, by Beyotime (1 mentions)
Malondialdehyde assay kit, by Beyotime (1 mentions)
Superoxide dismutase, by Beyotime (1 mentions)
Bicinchoninic acid protein assay kit, by Beyotime (1 mentions)
3 protocols using glutathione peroxidase
1
Antioxidant Activity of Herbal Extracts
2
Oxidative Stress Measurement Protocol
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The amount of oxidative stress generated was measured
as reported by Gu (23 (link)). We used 2ʹ,7ʹ-dichlorofluorescein
diacetate (DCFH-DA) to detect reactive oxygen species
(ROS) levels in the cells with an ELISA plate reader. Total
superoxide dismutase (SOD), glutathione peroxidase
(GDPs) and NADPH oxidase were with commercial kits
(Beyotime, Beijing, China).
as reported by Gu (23 (link)). We used 2ʹ,7ʹ-dichlorofluorescein
diacetate (DCFH-DA) to detect reactive oxygen species
(ROS) levels in the cells with an ELISA plate reader. Total
superoxide dismutase (SOD), glutathione peroxidase
(GDPs) and NADPH oxidase were with commercial kits
(Beyotime, Beijing, China).
3
Nasal Tissue Protein Analysis
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Nasal tissue proteins were extracted with a protein extraction kit (Beyotime, Shanghai, China). The levels of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were measured by superoxide dismutase, glutathione peroxidase and malondialdehyde assay kits according to manufacturer's instructions (Beyotime). Total protein concentration was determined by a bicinchoninic acid protein assay kit (Beyotime), and enzyme activity was standardized to milligram protein.
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