Aspc 1

Manufactured by Thermo Fisher Scientific

Sourced in United States, Australia, Germany

The AsPC-1 is a laboratory equipment product. It is a cell line derived from a human pancreatic adenocarcinoma. The core function of the AsPC-1 is to serve as a model system for cancer research and related applications.

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Lab products found in correlation

Aspc 1, by American Type Culture Collection (62 mentions) Panc 1, by Thermo Fisher Scientific (59 mentions) Panc 1, by American Type Culture Collection (52 mentions) Bxpc 3, by Thermo Fisher Scientific (51 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (42 mentions) Mia paca 2, by Thermo Fisher Scientific (39 mentions) Bxpc 3, by American Type Culture Collection (37 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (37 mentions) Mia paca 2, by American Type Culture Collection (35 mentions) Rpmi 1640, by Thermo Fisher Scientific (25 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (21 mentions) Cfpac 1, by Thermo Fisher Scientific (19 mentions) Capan 2, by Thermo Fisher Scientific (16 mentions) Sw1990, by American Type Culture Collection (15 mentions) Sw1990, by Thermo Fisher Scientific (14 mentions) Capan 2, by American Type Culture Collection (12 mentions) Capan 1, by Thermo Fisher Scientific (11 mentions) Penicillin, by Thermo Fisher Scientific (11 mentions) Streptomycin, by Thermo Fisher Scientific (11 mentions) Capan 1, by American Type Culture Collection (10 mentions)

100 protocols using aspc 1

Pancreatic Cell Line Cultivation and Lipid Assay

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Immortalized human pancreatic duct epithelial cell line HPDE6 and human pancreatic cancer cell line AsPC-1, BxPC-3, MIA PaCa-2 and PANC-1 were obtained from the American Type Culture Collection (ATCC). All cells were cultured at 37 °C in a humidified incubator with 5% CO₂ supply. Cells were grown in the following media: Keratinocyte Serum Free Medium (K-SFM) (Invitrogen) supplemented with 30 μg/ml BPE and 0.2 ng/ml rEGF for HPDE6 cell; DMEM high glucose (Invitrogen) supplemented with 10% FBS for PANC1 cell; RPMI 1640 (Invitrogen) supplemented with 10% FBS for AsPC-1, BxPC-3 and MIA PaCa2 cell. MIA PaCa-2 cell with stable expression of luciferase and mCherry fluorescent protein was obtained from In Vivo Therapeutics Core at Indiana University Simon Cancer Center (Indiana University, IN) and grown in DMEM supplemented with 10% FBS.
Chemicals including cholesteryl oleate, glyceryl trioleate, and simvastatin were purchased from Sigma-Aldrich. Avasimibe used in vitro and in vivo studies were purchased from Selleckchem.com. Human low-density lipoprotein (LDL) was purchased from Creative Laboratory Products (Indianapolis, IN) and conjugated with DiI by the authors. Lipoprotein-deficient Serum (LPDS) was purchased from Biomedical Technologies (Ward Hill, MA).

Li J., Gu D., Lee S.S., Song B., Bandyopadhyay S., Chen S., Konieczny S.F., Ratliff T.L., Liu X., Xie J, & Cheng J.X. (2016). Abrogating cholesterol esterification suppresses growth and metastasis of pancreatic cancer. Oncogene, 35(50), 6378-6388.

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Pancreatic Cancer Cell Line Characterization

Cited 2 times

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AsPC1, PANC1, BxPC3, H460, A549, and PC3 cells were purchased from the American Type Culture Collection (Manassas, VA). PANC1, BxPC3, H460, and PC3 were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium and AsPC1 in Dulbecco’s Modified Eagle Medium (DMEM), both supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100μg/ml streptomycin (Invitrogen). All cells were initially plated in their corresponding complete media for metabolic and cell death analyses. Cells were incubated at 37°C in a humidified incubator with 5% CO2. We avoid exposing carcinoma cell lines to heat-inactivated serum to maintain stable metabolic behavior.
The two PDAC lines, PANC1 and AsPC1, both resemble the majority genotype of pancreatic cancers in having activated KRAS and mutant p53 [56 (link)]. Moreover, CDKN2A is epigenetically down-regulated in both cell lines [57 (link), 58 (link)]. The CPI-613-resistant member of this pair, AsPC1, was isolated from a post-treatment metastasis [59 (link)]; whereas, CPI-613-sensitive PANC1 was isolated from an untreated primary tumor [56 (link), 60 (link)]. AsPC1 displays SMAD4 loss, as do the majority of clinical PDACs (whereas PANC1 retains SMAD4 expression) [61 (link)]. Collectively, these features are consistent with AsPC1 being a useful model for difficult-to-treat clinical PDAC cases more generally.

Guardado Rivas M.O., Stuart S.D., Thach D., Dahan M., Shorr R., Zachar Z, & Bingham P.M. (2022). Evidence for a novel, effective approach to targeting carcinoma catabolism exploiting the first-in-class, anti-cancer mitochondrial drug, CPI-613. PLoS ONE, 17(6), e0269620.

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Culturing Human Pancreatic Cell Lines

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The human PC cell lines AsPC-1, PANC-1, MIA Paca-2, and Capan-1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and KCLB (Korea Cell Line Bank, Seoul, South Korea). Human Umbilical Vein Endothelial Cells (HUVEC) are obtained from Lonza (Lonza, Basel, Switzerland). The non-cancerous immortalized human pancreatic ductal epithelial (HPDE) cell line was obtained from Joo Kyung Park (MD, Samsung Medical Center, Seoul, South Korea). MIA Paca-2 and PANC-1 cells were cultured in Dulbecco’s Modified Eagle Medium, while AsPC-1, and Capan-1 cells were cultured in RPMI-1640 (Gibco-Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum with 1% penicillin and streptomycin. Cells were maintained in a humidified 5% CO₂ atmosphere at 37 °C. HPDE cells were maintained in keratinocyte serum-free medium supplemented with epidermal growth factor and bovine pituitary extract (Gibco-Invitrogen).

Youn Y., Lee J.C., Kim J., Kim J.H, & Hwang J.H. (2020). Cdc6 disruption leads to centrosome abnormalities and chromosome instability in pancreatic cancer cells. Scientific Reports, 10, 16518.

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Pancreatic Cancer Cell Line Manipulation

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Human PC cells SW1990 were purchased from Procell (China) and cultured in Leibovitz's L-15 medium (Solarbio, China) with 10% FBS. PANC-1, AsPC-1, and BxPC-3 cell lines were purchased from iCell Bioscience (China). PANC-1 cells were treated with 10% FBS in DMEM medium. In addition, RPMI-1640 medium with 10% FBS was used for AsPC-1 and BxPC-3 cells. The above cells were cultured in an incubator at 37°C and 5% CO₂.
PANC-1 and AsPC-1 cells were used to silence or overexpress ITGBL1. For ITGBL1 downregulation, PANC-1 and AsPC-1 cells were transfected with short hairpin RNAs (shRNAs) specifically targeting ITGBL1 or the negative control (NC) for 48 h. For ITGBL1 overexpression, PANC-1 and AsPC-1 cells were transfected with ITGBL1 overexpression vector or empty vector for 48 h. For co-transfection, PANC-1 cells were co-transfected with ITGBL1 overexpression vector and JDP2 overexpression vector for 24 h. The transfections were mediated by Lipofectamine 3000 (Invitrogen, USA), according to the manufacturer's instruction.

Du T., Zhang K., Zhang Z., Guo A., Yu G, & Xu Y. (2022). ITGBL1 transcriptionally inhibited by JDP2 promotes the development of pancreatic cancer through the TGF-beta/Smad pathway. Brazilian Journal of Medical and Biological Research, 55, e11989.

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Pancreatic Cancer Tissue and Cell Line Characterization

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A total of 49 pairs of pancreatic cancer tissues and related cancer-adjacent normal tissues were obtained from patients who underwent surgical resection at the Southwest Hospital, Third Military Medical University. All the tumor tissues confirmed by pathology were preserved in liquid nitrogen. None of the patients had received chemotherapy or radiotherapy. This project was approved by the Ethical Committee of Third Military Medical University, China.
Aspc-1, Bxpc-3, Panc-1, and SW1990 cells were purchased from the American Type Culture Collection (Manassas, VA, USA), and the normal human pancreatic duct epithelial cells (hTERT-HPNE) were obtained from the Cell Bank Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). hTERT-HPNE, as well as Aspc-1, Bxpc-3 and SW1990 cells were grown in RPMI-1640 medium (Invitrogen, CA, USA) containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA). Panc-1 cells were cultured in Dulbecco's modified Eagle's medium (Invitrogen, Grand Island, NY, USA) containing 10% FBS.

You Y., Tan J., Gong Y., Dai H., Chen H., Xu X., Yang A., Zhang Y, & Bie P. (2017). MicroRNA-216b-5p Functions as a Tumor-suppressive RNA by Targeting TPT1 in Pancreatic Cancer Cells. Journal of Cancer, 8(14), 2854-2865.

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Cell Line Cultivation and Clinical Samples

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Cell lines: The PDAC cell lines Capan-1, Capan-2, MIA-Paca2, Panc-1, and Aspc-1, the embryonic kidney cell line HEK293, and NIH3T3 were purchased from American Type Culture Collection (ATCC, Rockville, Maryland). The PDAC cell lines PK-59 and KLM-1 were provided by the Cell Resource Center for Biomedical Research, Tohoku University (Sendai, Japan). All cells were cultured in appropriate media: RPMI-1640 (Sigma, St. Louis, MO) for Capan-1, Capan-2, Aspc-1, KLM-1, and PK-59 and Dulbecco's modified Eagle's medium (Invitrogen, Carlsbad, CA) for MIApaca-2, Panc-1, and NIH3T3. Each medium was supplemented with 10% fetal bovine serum (Cansera) and 1% antibiotic/antimycotic solution (Sigma). Cells were maintained at 37°C in an atmosphere of humidified air with 5% CO₂. Clinical samples (pancreatic cancer and normal pancreatic duct) were obtained from surgical specimens that were resected at Hokkaido University Hospital. Informed consent was obtained from patients, and the study was approved by the institutional review board of Hokkaido University Hospital (ID; 014-0224). Normal tissue sections were purchased from Biochain (Hayward, CA, USA).

Nakamura T., Katagiri T., Sato S., Kushibiki T., Hontani K., Tsuchikawa T., Hirano S, & Nakamura Y. (2016). Overexpression of C16orf74 is involved in aggressive pancreatic cancers. Oncotarget, 8(31), 50460-50475.

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Characterization of Pancreatic Cell Lines

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Immortalized human pancreatic duct epithelial cell line HPDE6 and human pancreatic cancer cell line AsPC-1, BxPC-3, MIA PaCa-2 and PANC-1 were obtained from the American Type Culture Collection (ATCC). All cells were cultured at 37 °C in a humidified incubator with 5% CO₂ supply. Cells were grown in the following media: Keratinocyte Serum Free Medium (Invitrogen, Carlsbad, CA, USA) supplemented with 30 μg/ml BPE and 0.2 ng/ml rEGF for HPDE6 cell; DMEM high glucose (Invitrogen) supplemented with 10% FBS for PANC-1 cell; RPMI 1640 (Invitrogen) supplemented with 10% FBS for AsPC-1, BxPC-3 and MIA PaCa-2 cells. MIA PaCa-2 cells with stable expression of luciferase and mCherry fluorescent protein was obtained from In Vivo Therapeutics Core at Indiana University Simon Cancer Center (Indiana University, IN) and grown in DMEM supplemented with 10% FBS.
Chemicals including cholesteryl oleate, glyceryl trioleate and simvastatin were purchased from Sigma–Aldrich (St Louis, MO, USA). Avasimibe used in vitro and in vivo studies were purchased from Selleckchem.com. Human LDL was purchased from Creative Laboratory Products (Indianapolis, IN, USA) and conjugated with DiI by the authors. Lipoprotein-deficient Serum was purchased from Biomedical Technologies (Ward Hill, MA, USA).

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Cultivation of Pancreatic Cell Lines

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Human PC cell lines (AsPC-1 and PANC-1) and a human pancreatic duct epithelial cell line (H6c7) were purchased from Beijing Beina Chuanglian Biotechnology Institute. A pancreatic cancer cell line (BxPC-3) was purchased from Wuhan Myhalic Biotechnological Co., Ltd. BxPC-3 and AsPC-1 cells were cultured in RPMI 1640 (Invitrogen, U.S.A.) containing 10% fetal bovine serum (FBS; HyClone), and PANC-1 and H6c7 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) with 10% FBS. All cell lines were maintained in a humidified incubator containing 5% CO₂ at 37°C.

Wang W., Pan H., Ren F., Chen H, & Ren P. (2022). Targeting ASCT2-mediated glutamine metabolism inhibits proliferation and promotes apoptosis of pancreatic cancer cells. Bioscience Reports, 42(3), BSR20212171.

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Modulating SNHG7 and miR-146b-5p in PC cells

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In total, four PC cell lines PANC-1, SW1990, BxPC-3 and AsPC-1, as well as the healthy pancreas ductal epithelial cell line HPDE were purchased from Tong Pai Technology. Cells were cultured in DMEM [Wokawi (Beijing) Biotechnology Co., Ltd.] supplemented with 10% FBS (Beijing BioDee Biotechnology Co., Ltd.) in a 5% CO₂ incubator at 37˚C.
Short hairpin RNA (shRNA) targeting SNHG7 (sh-SNHG7) and its negative control (sh-control), miR-146b-5p mimics (miR-146b-5p: 5'-UGAGAACUGAAUUCCAUAGGCU-3') and its control (miR-control: 5'-UUCUCCGAACGUGUCACGUTT-3'), miR-146b-5p inhibitor (5'-AGCCUAUGGAAUUCAGUUCUCA-3') and its control (inhibitor-control: 5'-CAGUACUUUUGUGUAGUACAA-3') were obtained from Shanghai GenePharma Co., Ltd. The sequences of SNHG7 (Accession: NR_024543.1) and Robo1 (Accession: GBYX01104758.1) were inserted into pcDNA vectors (Invitrogen; Thermo Fisher Scientific, Inc.) to construct overexpression plasmids, referred to as pcDNA-SNHG7 and Robo1, respectively. SW1990 and AsPC-1 cells were transfected with the constructed vectors or miRs (final concentration 50 µM) by using Lipofectamine^® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions 48 h after transfection, the cells were used for further assays.

Jian Y, & Fan Q. (2021). Long non-coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR-146b-5p/Robo1 axis. Experimental and Therapeutic Medicine, 21(4), 398.

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Culturing Pancreatic Adenocarcinoma Cell Lines

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The human pancreatic adenocarcinoma (PA) cell line Bxpc3, Aspc1 and MIAPaca2 were purchased from the American Type Culture Collection (ATCC, Manassas, VA). Aspc1 and MIAPaca2 cells were cultured in Dulbecco's Modified Eagle's Medium - high glucose (DMEM) with 10% fetal bovine serum (Invitrogen), 1% penicillin-streptomycin. Bxpc3 cells were cultured in RPMI-1640 medium (HyClone) with 10% fetal bovine serum and 1% penicillin-streptomycin and in presence of 5% CO₂.

Kumar S.A., Thakur C., Li L., Cui H, & Chen F. (2017). Pathological and prognostic role of mdig in pancreatic cancer. Genes & Cancer, 8(7-8), 650-658.

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