Af3293

Manufactured by Affinity Biosciences

Sourced in United States, China

AF3293 is a high-performance electrophoresis system designed for molecular biology and biochemistry applications. It provides a reliable and consistent platform for the separation and analysis of DNA, RNA, and protein samples.

Automatically generated - may contain errors

Lab products found in correlation

Af6294, by Affinity Biosciences (3 mentions) Stattic, by Apexbio (1 mentions) Peg300, by Solarbio (1 mentions) Lipopolysaccharides (lps), by Cloud-Clone (1 mentions) Af1234, by Beyotime (1 mentions) P stat3, by Affinity Biosciences (1 mentions) Stat3, by Beyotime (1 mentions) C myc, by Affinity Biosciences (1 mentions) Cyclin d1, by Affinity Biosciences (1 mentions) Bcl 2, by Beyotime (1 mentions) β tubulin, by Beyotime (1 mentions) Af1216, by Beyotime (1 mentions) Hrp conjugated goat anti rabbit igg, by CWBIO (1 mentions) Hrp conjugated goat anti mouse igg, by CWBIO (1 mentions) Docetaxel, by Apexbio (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions) Dimethyl sulfoxide (dmso), by Solarbio (1 mentions) Lipopolysaccharides (lps), by Solarbio (1 mentions) Bay 11 7082, by Beyotime (1 mentions) 3 3 diaminobenzidine (dab), by ZSGB-BIO (1 mentions)

4 protocols using af3293

Molecular Mechanisms of LPS-Induced Inflammation

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Reagents: ampicillin (A8180-2, Solarbio, China); colistin (R002831, Rhawn, China); neomycin and vancomycin (N814740 and V820413, Macklin, China); LPS (L8880, Solarbio, China); BAY-11-7082 (S1523, Beyotime, China); Stattic (A2224, APExBIO, USA); Docetaxel (A4394, APExBIO, USA); DMSO (D8370, Solarbio, China); PEG300 (IP9020, Solarbio, China); Tween-80 (T8360, Solarbio, China); DAPI (C0065, Solarbio, China); DAB (ZLI-9018, ZSGB-BIO, China).
Antibodies: Ki-67 (ab15580, abcam, UK); LPS (MAB526Ge22, Cloud-Clone, China); p65 (AF1234, Beyotime, China); p-p65 (Ser536) (AF2006, Affinity, USA); p-STAT3 (Tyr705) (AF3293, Affinity, USA); STAT3 (AF1492, Beyotime, China); Antibody-IL6 (AF-406-SP, R&D, USA); C-myc (AF0358, Affinity, USA); Cyclin D1 (AF0931, Affinity, USA); Bcl-2 (AF6285, Beyotime, China); Survivin (AF6017, Affinity, USA); β-actin (AF5003, Beyotime, China); β-tubulin (AF1216, Beyotime, China). Second antibodies: HRP-conjugated goat anti-rabbit IgG (cw0103s, CWBIO, China); HRP-conjugated goat anti-mouse IgG (cw0102s, CWBIO, China); fluorescent Cy3-conjugated goat anti-rabbit IgG (BA1032, BOSTER, China).

Zhong W., Wu K., Long Z., Zhou X., Zhong C., Wang S., Lai H., Guo Y., Lv D., Lu J, & Mao X. (2022). Gut dysbiosis promotes prostate cancer progression and docetaxel resistance via activating NF-κB-IL6-STAT3 axis. Microbiome, 10, 94.

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Western Blot Analysis of Protein Expression

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Total protein lysates were extracted with cell lysis buffer (P0013, Beyotime) containing a protease inhibitor (ST506, Beyotime) and quantified with a BCA assay kit (P0011, Beyotime). Protein samples were loaded on SDS‒PAGE gels and transferred onto polyvinylidene difluoride membranes. After being blocked in 5% BSA for 1 h at room temperature, the membranes were hybridized with primary antibodies against JMJD1C (1:1000; A20153, ABclonal, Shanghai, China), HK2 (1:1000; A0994, ABclonal), PGK1 (1:1000; A12686, ABclonal), LDHA (1:1000; A0861, ABclonal), p-STAT3 (1:1000; AF3293, Affinity), STAT3 (1:1000; AF6294, Affinity), cyclin D1 (1:1000; A19038, ABclonal), CDK4 (1:1000; A11136, ABclonal) or β-actin (1:1000; sc-47778, Santa Cruz, Dallas, TX, USA) overnight at 4 °C, followed by goat anti-rabbit IgG (1:5000; A0208, Beyotime) or goat anti-mouse IgG (1:5000; A0216, Beyotime) secondary antibody incubation for 45 min at 37 °C. The protein signals were visualized by an enhanced chemiluminescence system (P0018, Beyotime) and quantified using Gel-Pro-Analyzer Software (WD-9413B, Beijing Liuyi Biotechnology Co., Ltd., Beijing, China).

Zhang C., Sun Y., Guo Y., Xu J, & Zhao H. (2023). JMJD1C promotes smooth muscle cell proliferation by activating glycolysis in pulmonary arterial hypertension. Cell Death Discovery, 9, 98.

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Ferroptosis Regulators and Cellular Signaling

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Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China). Vitamin E was purchased from Beyotime (Shanghai, China). Rabbit polyclonal anti-transferrin receptor antibody (TFRC, AF5343, 1 : 1000), rabbit polyclonal anti-divalent metal transporter-1 antibody (DMT1, DF12740. 1 : 1000), rabbit polyclonal anti-ferritin light chain antibody (FTL, DF6604, 1 : 1000), rabbit polyclonal anti-ferritin heavy chain antibody (FTH, DF6278, 1 : 1000), rabbit polyclonal anti-SLC7A11 antibody (DF12509, 1 : 1000), rabbit polyclonal anti-P53 antibody (AF0879, 1 : 1000), rabbit polyclonal anti-STAT3 antibody (AF6294, 1 : 1000), rabbit polyclonal anti-p-STAT3 (705) antibody (AF3293, 1 : 1000), and rabbit polyclonal anti-glutathione peroxidase-4 antibody (GPX4, DF6701, 1 : 1000) were purchased from Affinity Bioscience (China). HRP goat anti-rabbit IgG was purchased from Earthox (USA).

Luo Y., Gao X., Zou L., Lei M., Feng J, & Hu Z. (2021). Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells. Oxidative Medicine and Cellular Longevity, 2021, 1783485.

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Cardiac TLR4, STAT3 Protein Analysis

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Toll-like receptor 4 (TLR4) (Affinity Biosciences Cat# AF7017, RRID: AB_2835322; Affinity Biosciences, Cincinnati, OH, USA), total signal transducer and activator of transcription 3 (T-STAT3) (Affinity Biosciences Cat# AF6294, RRID: AB_2835144) and its phosphorylated (Affinity Biosciences Cat# AF3293, RRID: AB_2810278) form in heart muscle were evaluated by Western blotting. Samples were homogenized in a lysis buffer, sonicated, and centrifuged. Protein concentration was determined using a bicinchoninic acid (BCA) protein quantitation assay. Protein (40 μg) were separated by 10% SDS-PAGE, transferred to polyvinylidene difluoride (PVDF) membranes, blocked with 5% bovine serum albumin (BSA) [in Tris-buffered saline with 0.1% Tween 20 (TBST)] for 2 h at room temperature and then incubated with primary antibodies overnight at 4°C. The membranes were then washed and incubated with secondary horseradish peroxidase-conjugated antibodies for 1 h at room temperature. After washing with TBST, bands were detected using the enhanced chemifluorescence reagent. The intra- and inter-assay variation was tested in GAPDH and β-actin, and western blotting efficiency of the two reference proteins were similar and constant. Band intensity was quantified by scanning densitometry using Image J software and normalized to GAPDH (Affinity Biosciences Cat# AF7021, RRID: AB_2839421).

Zhang Q.Q., Li G.Q., Zhong Y., Wang J., Wang A.N., Zhou X, & Mao X.M. (2020). Empagliflozin improves chronic hypercortisolism-induced abnormal myocardial structure and cardiac function in mice. Therapeutic Advances in Chronic Disease, 11, 2040622320974833.

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